Decontamination of a polychlorinated biphenyls-contaminated soil by phytoremediation-assisted bioaugmentation

Autor: Jean-Yves Cornu, Karine Jézéquel, Marc Lollier, Thierry Lebeau, C. Secher, Laurence Amalric
Přispěvatelé: Equipe Dépollution Biologique des Sols, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG), Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2013
Předmět:
Festuca
Bioaugmentation
fescue
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Environmental Engineering
Burkholderia
Burkholderia xenovorans
Biomass
Bioengineering
010501 environmental sciences
Biology
01 natural sciences
Microbiology
03 medical and health sciences
Animal science
Soil Pollutants
Environmental Chemistry
PCBs
[INFO.INFO-BT]Computer Science [cs]/Biotechnology
bioaugmentation
Environmental Restoration and Remediation
Soil Microbiology
TTGE
030304 developmental biology
0105 earth and related environmental sciences
2. Zero hunger
0303 health sciences
Ecology
food and beverages
Human decontamination
15. Life on land
biology.organism_classification
Polychlorinated Biphenyls
Pollution
Soil contamination
LB400
6. Clean water
Phytoremediation
Biodegradation
Environmental
real-time PCR
Festuca arundinacea
Bacteria
Zdroj: Biodegradation
Biodegradation, Springer Verlag, 2013, 24 (4), pp.549-562. ⟨10.1007/s10532-013-9625-6⟩
ISSN: 1572-9729
0923-9820
DOI: 10.1007/s10532-013-9625-6
Popis: International audience; A 70 day pot experiment was conducted for the cleaning-up of a PCBs-contaminated soil (104 mg kg(-1) soil DW) using bioaugmentation with Burkholderia xenovorans LB400 (LB400) assisted or not by the use of tall fescue (Festuca arundinacea). The total cultivable bacteria of the soil were higher with the presence of plants. Real-time PCR showed that LB400 (targeting 16S-23S rRNA ITS) survived with abundance related to total bacteria (targeting 16S rRNA) being higher with fescue (up to a factor of three). Bioaugmentation had a positive effect on fescue biomass and more specifically on roots (by a factor of three). PCB dissipation (sum of congeners 28, 52, 101, 118, 153, 180) averaged 13 % (bioaugmented-planted) up to 32 % (non bioaugmented-planted), without any significant difference between treatments. Basically our results demonstrated that indigenous bacteria were able to dissipate PCBs (26.2 % dissipation). PCB dissipation was not related to the abundance of LB400 or to the total bacterial counts. Bioaugmentation or fescue altered the structure of the bacterial community of the soil, not the combination of both. Principal component analysis showed that bioaugmentation tended to improve the control of the process (lower variability in PCB dissipation). Opposite to that bioaugmentation increased the variability of the structure of the bacterial community.
Databáze: OpenAIRE
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