A study of the effect of salicylic acetic acid on a lymphocyte cell model of cellular activation and proliferation
| Autor: | Eduardo Muñoz, Concha Lucena, Enrique Aranda Aguilar, Juan de la Haba-Rodriguez, Antonio Macho, Auxiliadora Gómez, Marco A. Calzado |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Lymphocyte Lymphocyte Activation medicine.disease_cause Models Biological Flow cytometry Enterotoxins chemistry.chemical_compound Acetic acid medicine Humans Lymphocytes IL-2 receptor Receptor Cells Cultured Cell Proliferation Aspirin medicine.diagnostic_test Cell Cycle Receptors Interleukin-2 DNA Flow Cytometry Cell biology medicine.anatomical_structure Oncology Biochemistry chemistry Fluorescent Antibody Technique Direct Thymidine Carcinogenesis Alpha chain |
| Zdroj: | Cancer Letters. 231:257-261 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/j.canlet.2005.02.014 |
| Popis: | Salicylic acetic acid (SAA) is a drug that has formed part of the treatment of many diseases for many years. Its anti-inflammatory activity is well known, but recently its possible role in the interference in the oncogenesis mechanisms has become apparent. With the aim of supporting these yet preliminary observations, we studied the effect of salicylic acetic acid on a cellular activation and proliferation model. We used lymphocytes obtained from peripheral blood, which were later exposed to cellular activation and proliferation stimulus by the SEB antigen. Lymphocyte activation was determined by direct immunoflourescence through expression of the receptor IL-2 (CD25) alpha chain and proliferation through the incorporation of tritiated thymidine to the DNA in synthesis together with the determination of the cellular cycle by flow cytometry. We found that both processes, activation and proliferation, are inhibited by increasing doses of SAA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect