Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
| Autor: | Noemia S. Lima, Ricardo Galler, Juliana Ribeiro dos Santos, Patrícia Cristina da Costa Neves, David I. Watkins, Marlon G. Veloso de Santana, Myrna C. Bonaldo, Alexandre Dos Santos |
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| Rok vydání: | 2014 |
| Předmět: |
viruses
Genetic Vectors Molecular Sequence Data Yellow Fever 17D virus Gene Products gag Heterologous HIV Infections Biology Recombinant virus medicine.disease_cause Article Insert (molecular biology) Virus Viral vector law.invention Mice law Virology medicine Animals Humans Amino Acid Sequence AIDS Vaccines Mice Inbred BALB C Base Sequence SIV gag IRES Genetic stability Group-specific antigen Simian immunodeficiency virus Molecular biology 3. Good health SIV Gag Recombinant DNA Cytokines Nucleic Acid Conformation Female Simian Immunodeficiency Virus Yellow fever virus |
| Zdroj: | Virology. :202-211 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2014.01.017 |
| Popis: | We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness. |
| Databáze: | OpenAIRE |
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