The effects of ABCG2 on the viability, proliferation and paracrine actions of kidney side population cells under oxygen-glucose deprivation
| Autor: | Qiu-Hong Meng, De-Wei Du, Hua Han, Ji-Feng Sun, Hong-Bao Liu, Jian-Bo Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Blood Glucose
Male Stromal cell Cell Survival medicine.medical_treatment Blotting Western Proliferation Apoptosis Stem cells ischemia Biology Kidney Paracrine signalling chemistry.chemical_compound Mice Side population medicine ATP Binding Cassette Transporter Subfamily G Member 2 Animals Cells Cultured Cell Proliferation Reverse Transcriptase Polymerase Chain Reaction hypoxia Growth factor Cell Cycle General Medicine Cell biology Vascular endothelial growth factor carbohydrates (lipids) Oxygen chemistry nervous system Paracrine Hepatocyte growth factor ATP-Binding Cassette Transporters Stem cell oxygen-glucose deprivation medicine.drug Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Bcrp1/ABCG2 is exclusively expressed in side population (SP) cells, however, it has not been fully elucidated whether it has an impact on the viability, proliferation and paracrine actions in kidney SP cells under oxygen-glucose deprivation (OGD) followed by reoxygenation. In this study, we found that 2-h OGD did not injure SP cells (sub-lethal OGD) but induced SP cells proliferation 48 and 72 h after reoxygenation; whereas 4-h OGD markedly injured the cells (lethal OGD) and led to apoptosis 24-72 h after reoxygenation. Fumitremorgin C, an inhibitor of ABCG2, attenuated both the proliferation and viability of SP cells. Sub-lethal and lethal OGD induced the increase in the secretion of vascular endothelial growth factor, insulin-like growth factor 1, hepatocyte growth factor, and stromal cell-derived factor-1α in kidney SP cells, which was inhibited by Fumitremorgin C. Collectively, these findings provide evidence for a crucial role for the ABCG2 expression in the viability, proliferation and paracrine actions of kidney SP cells after OGD. |
| Databáze: | OpenAIRE |
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