Prenatal alcohol exposure alters the cerebral cortex proteome in weanling rats
| Autor: | Lorena Canales, Rachel Neal, Jing Chen, Caitlin Gambrell |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Proteome Offspring Weanling Ubiquitin-conjugating enzyme Carbohydrate metabolism Biology Toxicology Article Rats Sprague-Dawley chemistry.chemical_compound Pregnancy Cortex (anatomy) Internal medicine Reflex medicine Animals Cerebral Cortex Ethanol Rats Cytoskeletal Proteins medicine.anatomical_structure Endocrinology Animals Newborn chemistry Proteasome Motor Skills Cerebral cortex Prenatal Exposure Delayed Effects Female Protein Processing Post-Translational |
| Zdroj: | Reproductive Toxicology. 39:69-75 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2013.04.003 |
| Popis: | Maternal consumption of alcohol during pregnancy impairs neurodevelopment in offspring. Utilizing a rodent model of continuous moderate dose alcohol exposure throughout gestation [gestation day 1 (GD1)-GD22; BAC ~70 mg/dL], the impact of developmental alcohol exposure on juvenile cerebral cortex protein abundances was determined. At weaning, cerebral cortex tissue was collected from pups for 2D SDS-PAGE based proteome analysis with statistical analysis by Partial Least Squares-Discriminant Analysis (PLS-DA). Gestational alcohol exposure increased the abundance of post-translationally modified forms of cytoskeletal proteins and the abundance of proteins within the small molecule biochemistry (includes glucose metabolism) pathway and proteosome processing pathways though ubiquitin conjugating enzymes and chaperones were decreased in abundance. In weanling offspring exposed prenatally to alcohol, alterations in cytoskeletal protein post-translational modifications were noted. Increased abundance of proteins from the small molecule biochemistry pathway, which includes glucose metabolism, and proteosome processing pathways were also noted. Decreased abundances of ubiquitin conjugating enzyme and chaperone protein were noted in the cerebral cortex of these offspring. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect