SD Bioline malaria antigen Pf (HRP-2/pLHD) for assessing efficacy of artemisinin combination therapy against Plasmodium falciparum in pediatric patients in the Democratic Republic of the Congo
| Autor: | Mgaywa Gilbert Mjungu Damas Magafu, Jose Gaby Tshikuka Mulumba, Naoko Shimizu Magafu, Reginald Matchaba-Hove, Roy Tapera, Albert Lukuka Kilauzi, Jean Jacques Muyembe Tamfum |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Combination therapy Plasmodium falciparum Drug Resistance malaria Antigens Protozoan drc Drug resistance Sensitivity and Specificity agpf(hrp-2/pldh) rdt Antimalarials Predictive Value of Tests Internal medicine Positive predicative value parasitic diseases Humans Medicine Malaria Falciparum Artemisinin artemisinin combination therapy lcsh:R5-920 Rapid diagnostic test biology Diagnostic Tests Routine business.industry Research lcsh:Public aspects of medicine Infant lcsh:RA1-1270 General Medicine biology.organism_classification medicine.disease Artemisinins Surgery Child Preschool Predictive value of tests Democratic Republic of the Congo Drug Therapy Combination Female Malaria AgPf(HRP-2/pLDH) RDT artemisinin combination therapy Democratic Republic of the Congo lcsh:Medicine (General) business Malaria Follow-Up Studies medicine.drug |
| Zdroj: | Pan African Medical Journal; Vol 22, No 1 (2015) The Pan African Medical Journal, Vol 22, Iss 304 (2015) The Pan African Medical Journal |
| ISSN: | 1937-8688 |
| Popis: | Introduction : The emergence of Plasmodium falciparum resistance to artemisinin combination therapy (ACT) is a worrying development. It calls for close surveillance to monitor the efficacy of the drugs. The objectives of this study were to determine the performance of SD Bioline malaria AgPf(HRP-2/pLDH) 3 band Rapid Diagnostic Test (RDT) against Giemsa-stained blood smear and evaluate the suitability of this test in assessing the therapeutic efficacy of ACT in pediatric malaria patients in the Democratic Republic of the Congo (DRC). Methods : Five hundred and one patients with malaria symptoms were screened for P. falciparum in Kinshasa, DRC. Of the 166 patients who tested positive for P. falciparum at recruitment (day 0), 103 consented to participate in this study and were followed up and retested for P. falciparum on day 3, day 7, day 14, day 21 and day 28. Results : Sensitivity and specificity of the test were significantly high on day 0 and so were their positive and negative predictive values. Higher proportions of false positive cases were observed on the HRP-2 band irrespective of patient parasite densities during the follow up but these were barely seen on the pLDH band. Some patients turned positive during follow up but pLDH readings remained consistent with blood smear readings. Conclusion : SD Bioline malaria AgPf(HRP-2/pLDH) RDT demonstrated high performance in DRC. Thus, the test can be employed to assess the efficacy of ACT in pediatric malaria patients and prioritize areas that require the deployment of advanced testing like polymerase chain reaction (PCR). Key words : Malaria, AgPf(HRP-2/pLDH) RDT, artemisinin combination therapy, Democratic Republic of the Congo |
| Databáze: | OpenAIRE |
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