Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics

Autor: Satoru Takeo, Ji-Yun Lee, Sung-Jong Hong, Eun-Taek Han, Sung-Tae Hong, Takafumi Tsuboi, Won Gi Yoo, Banchob Sripa, Pyo Yun Cho, Jae Wan Jung, Tae Im Kim, Jong-Yil Chai, Jhang Ho Pak, Ho Woo Nam, Kwon-Soo Ha, Tong-Soo Kim, Jin-Ho Song
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Serum Proteins
Physiology
Flatworms
RC955-962
Artificial Gene Amplification and Extension
Protein Synthesis
Biochemistry
Polymerase Chain Reaction
law.invention
0302 clinical medicine
Medical Conditions
law
Raw Foods
Immune Physiology
Arctic medicine. Tropical medicine
Medicine and Health Sciences
Polymerase chain reaction
Proteogenomics
Clonorchis sinensis
Immune System Proteins
Clonorchis
biology
Fishes
Eukaryota
Chemical Synthesis
Recombinant Proteins
Infectious Diseases
Helminth Infections
Point-of-Care Testing
Clonorchiasis
Protein microarray
Recombinant DNA
Public aspects of medicine
RA1-1270
Research Article
Neglected Tropical Diseases
Antigenicity
Biosynthetic Techniques
030231 tropical medicine
Immunology
Antibodies
Helminth
Enzyme-Linked Immunosorbent Assay
Cross Reactions
Research and Analysis Methods
Trematodes
Sensitivity and Specificity
03 medical and health sciences
Antigen
Helminths
medicine
Parasitic Diseases
Animals
Humans
Serologic Tests
Antigens
Molecular Biology Techniques
Molecular Biology
Opisthorchis
Public Health
Environmental and Occupational Health
Organisms
Biology and Life Sciences
Proteins
medicine.disease
biology.organism_classification
Tropical Diseases
Virology
Invertebrates
030104 developmental biology
Antigens
Helminth
Immunoglobulin G
Zoology
Foodborne Trematodiases
Cloning
Zdroj: PLoS Neglected Tropical Diseases, Vol 14, Iss 12, p e0008998 (2020)
PLoS Neglected Tropical Diseases
ISSN: 1935-2735
1935-2727
Popis: Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.
Author summary Human clonorchiasis is a parasitic disease caused by the Chinese liver fluke, Clonorchis sinensis. Humans are infected through eating raw freshwater fishes carrying C. sinensis metacercariae, the encysted larvae. They excyst in the duodenum, move into the liver via bile duct and grow to adult worms. Excretory-secretory products of the worm damage the liver causing various inflammatory pathological changes and may lead to bile duct cancer. Although there exists an anthelmintic choice praziquantel to kill the fluke, emphasis is placed on early diagnosis and prevention before the infection becomes disease. Microscopic stool examination is the standard diagnostic method but is cumbersome and time consuming. Blood serum antibodies from clonorchiasis patients could provide a simple and fast diagnosis. However, antibody detecting diagnostics developed so far have a low specificity and sensitivity. In the present study we selected 607 antigenic candidate proteins from the genomic database and synthesized them through an integrated high-throughput proteogenomic tools. We identified several antigenic proteins and evaluated their diagnostic potential for clonorchiasis. One of them, CsAg17, showed a high sensitivity and specificity. This antigen deserves development of point-of-care serodiagnostics for C. sinensis infections.
Databáze: OpenAIRE
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