Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17β and progesterone concentrations in preovulatory follicular fluid
| Autor: | Yosuke Gunji, Kazuma Sumitomo, Mitsugu Hishinuma, Chihiro Ogino, Ryo Nishimura, Minami Matsuo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cultural Studies medicine.medical_specialty Insemination lcsh:Agriculture 03 medical and health sciences 0302 clinical medicine Internal medicine lcsh:Zoology medicine lcsh:QL1-991 Blastocyst lcsh:Science lcsh:SF1-1100 030219 obstetrics & reproductive medicine Chemistry lcsh:S Religious studies Embryo Estradiol 17 β Follicular fluid Sperm In vitro In vitro maturation 030104 developmental biology Endocrinology medicine.anatomical_structure lcsh:Q lcsh:Animal culture |
| Zdroj: | Archives Animal Breeding, Vol 60, Pp 385-390 (2017) |
| ISSN: | 2363-9822 |
| Popis: | The objective of the article is to evaluate the effect of three-step in vitro maturation (IVM) culture system imitating estradiol-17β (E2) and progesterone (P4) concentrations in preovulatory follicles on in vitro bovine embryo production. The cumulus–oocyte complexes (COCs) were collected from follicles (2 to 8 mm in diameter) of bovine ovaries obtained from a local slaughterhouse. For IVM, the COCs were cultured for 22 h in a three-step system: (1) culture in medium 199, containing 700 ng mL−1 E2 and 50 ng mL−1 P4, for 5 h, followed by the medium containing 150 ng mL−1 E2 and 150 ng mL−1 P4 for 11 h, and then the medium containing 20 ng mL−1 E2 and 300 ng mL−1 P4 for 6 h (EP group); (2) culture in the medium containing 700 ng mL−1 E2 for 5 h, followed by the medium containing 150 ng mL−1 E2 for 11 h, and then the medium containing 20 ng mL−1 E2 for 6 h (E group); or (3) culture in the medium containing 50 ng mL−1 P4 for 5 h, followed by the medium containing 150 ng mL−1 P4 for 11 h, and then the medium containing 300 ng mL−1 P4 for 6 h (P group). The COCs were cultured in the medium containing 1000 ng mL−1 E2 for 22 h (control group). After IVM, the COCs were co-incubated with sperm and further cultured. At 48 h after insemination, the cleavage rate of embryos was not different among the groups. At 192 h after insemination, the blastocyst formation rate of EP group was significantly higher than that of the other groups. The total cell number of blastocysts did not differ among the groups. In conclusion, these results demonstrate that the three-step IVM culture system of bovine oocytes imitating temporal changes of E2 and P4 concentrations in preovulatory follicular fluid improves the developmental potential of embryos in vitro. |
| Databáze: | OpenAIRE |
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