Long Noncoding RNA HCAL Facilitates the Growth and Metastasis of Hepatocellular Carcinoma by Acting as a ceRNA of LAPTM4B
| Autor: | Qinliang Fang, Zhao Li, He-Qiang Qi, Fuqiang Wang, Sen Zheng, Chengrong Xie, Jie Lv, Sheng Zhang, Xiaomin Wang, Zhenyu Yin, Fei Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biology Bioinformatics Article Metastasis 03 medical and health sciences 0302 clinical medicine LAPTM4B Downregulation and upregulation Drug Discovery microRNA medicine Gene silencing competing endogenous RNA heptatocellular carcinoma long noncoding RNA Competing endogenous RNA Microarray analysis techniques lcsh:RM1-950 medicine.disease Long non-coding RNA digestive system diseases 030104 developmental biology lcsh:Therapeutics. Pharmacology 030220 oncology & carcinogenesis Cancer research Molecular Medicine |
| Zdroj: | Molecular Therapy: Nucleic Acids, Vol 9, Iss C, Pp 440-451 (2017) Molecular Therapy. Nucleic Acids |
| ISSN: | 2162-2531 |
| Popis: | Long noncoding RNAs (lncRNAs) are a new class of regulatory noncoding RNAs. Emerging evidences indicate that lncRNAs play a critical role in the development of hepatocellular carcinoma (HCC). Although several lncRNAs have been annotated, the association of most lncRNAs with HCC is unknown. In this study, we investigated lncRNA alterations in HCC by performing lncRNA microarray analysis. We identified a novel lncRNA called HCC-associated lncRNA (HCAL) that was highly expressed in HCC tissues. HCAL upregulation was clinically associated with poor differentiation, intravascular cancer embolus, and decreased survival of patients with HCC. HCAL silencing significantly inhibited the growth and metastasis of HCC cells both in vitro and in vivo. Interestingly, transcriptome-sequencing analysis of HCAL-knockdown cells showed alterations in some cancer-related pathways. Mechanistically, HCAL directly interacted with and functioned as a sponge for microRNAs such as miR-15a, miR-196a, and miR-196b to modulate LAPTM4B expression. Taken together, our findings suggest the presence of a novel lncRNA-miRNA-mRNA regulatory network, i.e., the HCAL-miR-15a/miR-196a/miR-196b-LAPTM4B network, in HCC and indicate that HCAL may be a potential target for treating HCC. |
| Databáze: | OpenAIRE |
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