MicroRNA Sequencing Revealed Citrus Adaptation to Long-Term Boron Toxicity through Modulation of Root Development by miR319 and miR171
Autor: | Lin Xiongjie, Li-Song Chen, Xian-da Wang, Jing-Hao Huang, Ling-Yuan Zhang, Guo-Cheng Fan |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Citrus Adaptation Biological Plant Development Biology 01 natural sciences Plant Roots Catalysis Article Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Gene Expression Regulation Plant MYB Physical and Theoretical Chemistry stem cell maintenance Molecular Biology Gene Lateral root formation lcsh:QH301-705.5 Spectroscopy Illumina dye sequencing Phylogeny boron toxicity Boron MicroRNA sequencing Gene Expression Profiling Organic Chemistry Computational Biology food and beverages General Medicine Computer Science Applications Cell biology microRNAs 030104 developmental biology Phenotype lcsh:Biology (General) lcsh:QD1-999 lateral root formation Toxicity RNA Interference Transcription Factor Gene Citrus × sinensis 010606 plant biology & botany |
Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 6 International Journal of Molecular Sciences, Vol 20, Iss 6, p 1422 (2019) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms20061422 |
Popis: | Boron (B) toxicity in Citrus is a common physiological disorder leading to reductions in both productivity and quality. Studies on how Citrus roots evade B toxicity may provide new insight into plant tolerance to B toxicity. Here, using Illumina sequencing, differentially expressed microRNAs (miRNAs) were identified in B toxicity-treated Citrus sinensis (tolerant) and C. grandis (intolerant) roots. The results showed that 37 miRNAs in C. grandis and 11 miRNAs in C. sinensis were differentially expressed when exposed to B toxicity. Among them, miR319, miR171, and miR396g-5p were confirmed via 5&prime RACE and qRT-PCR to target a myeloblastosis (MYB) transcription factor gene, a SCARECROW-like protein gene, and a cation transporting ATPase gene, respectively. Maintenance of SCARECROW expression in B treated Citrus roots might fulfill stem cell maintenance, quiescent center, and endodermis specification, thus allowing regular root elongation under B-toxic stress. Down-regulation of MYB due to up-regulation of miR319 in B toxicity-treated C. grandis roots might decrease the number of root tips, thereby dramatically changing root system architecture. Our findings suggested that miR319 and miR171 play a pivotal role in Citrus adaptation to long-term B toxicity by targeting MYB and SCARECROW, respectively, both of which are responsible for root growth and development. |
Databáze: | OpenAIRE |
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