MicroRNA Sequencing Revealed Citrus Adaptation to Long-Term Boron Toxicity through Modulation of Root Development by miR319 and miR171

Autor: Lin Xiongjie, Li-Song Chen, Xian-da Wang, Jing-Hao Huang, Ling-Yuan Zhang, Guo-Cheng Fan
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Citrus
Adaptation
Biological
Plant Development
Biology
01 natural sciences
Plant Roots
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
Gene Expression Regulation
Plant
MYB
Physical and Theoretical Chemistry
stem cell maintenance
Molecular Biology
Gene
Lateral root formation
lcsh:QH301-705.5
Spectroscopy
Illumina dye sequencing
Phylogeny
boron toxicity
Boron
MicroRNA sequencing
Gene Expression Profiling
Organic Chemistry
Computational Biology
food and beverages
General Medicine
Computer Science Applications
Cell biology
microRNAs
030104 developmental biology
Phenotype
lcsh:Biology (General)
lcsh:QD1-999
lateral root formation
Toxicity
RNA Interference
Transcription Factor Gene
Citrus × sinensis
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 6
International Journal of Molecular Sciences, Vol 20, Iss 6, p 1422 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20061422
Popis: Boron (B) toxicity in Citrus is a common physiological disorder leading to reductions in both productivity and quality. Studies on how Citrus roots evade B toxicity may provide new insight into plant tolerance to B toxicity. Here, using Illumina sequencing, differentially expressed microRNAs (miRNAs) were identified in B toxicity-treated Citrus sinensis (tolerant) and C. grandis (intolerant) roots. The results showed that 37 miRNAs in C. grandis and 11 miRNAs in C. sinensis were differentially expressed when exposed to B toxicity. Among them, miR319, miR171, and miR396g-5p were confirmed via 5&prime
RACE and qRT-PCR to target a myeloblastosis (MYB) transcription factor gene, a SCARECROW-like protein gene, and a cation transporting ATPase gene, respectively. Maintenance of SCARECROW expression in B treated Citrus roots might fulfill stem cell maintenance, quiescent center, and endodermis specification, thus allowing regular root elongation under B-toxic stress. Down-regulation of MYB due to up-regulation of miR319 in B toxicity-treated C. grandis roots might decrease the number of root tips, thereby dramatically changing root system architecture. Our findings suggested that miR319 and miR171 play a pivotal role in Citrus adaptation to long-term B toxicity by targeting MYB and SCARECROW, respectively, both of which are responsible for root growth and development.
Databáze: OpenAIRE
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