Estrogenic and antiproliferative properties of soy sapogenols in human breast cancer cells in vitro
| Autor: | J.C Rowlands, Mark A. Berhow, T.M Badger |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Estrogen receptor Gene Expression Breast Neoplasms Phytoestrogens Biology Toxicology chemistry.chemical_compound Internal medicine medicine Tumor Cells Cultured Humans Electrophoretic mobility shift assay Estrogens Non-Steroidal RNA Messenger Oleanolic Acid skin and connective tissue diseases Anticarcinogen Fulvestrant Dose-Response Relationship Drug Estradiol Cell growth Plant Extracts Estrogen Antagonists Biological activity Estrogens General Medicine Saponins Blotting Northern Molecular biology Isoflavones In vitro Endocrinology chemistry Receptors Estrogen Cancer cell Female Plant Preparations Soybeans hormones hormone substitutes and hormone antagonists Cell Division Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 40(12) |
| ISSN: | 0278-6915 |
| Popis: | Two soy sapogenols, soyasapogenol A (SA) and soyasapogenol B (SB) were tested for their estrogenic activities in estrogen responsive MCF-7 or estrogen-insensitive MDA-MB-231 (MDA) human breast cancer cells. SB and SA had differential actives on cell proliferation with 10 microM SB being growth inhibitory to MDA cells with no significant effect at any concentration on MCF-7 cells. SA also inhibited MDA cell proliferation at 10 micro, but at this same dose stimulated a 2.5-fold increase in MCF-7 proliferation. SA (0.1-10 microM) induced pS2 mRNA levels and the induction was blocked by co-treatment of cells with the anti-estrogen ICI 182,780. SA also induced the formation of an ER-ERE DNA complex measured by electrophoretic mobility shift assay. In summary, these results show that soyasapogenol A is estrogenic, whereas soyasapogenol B is growth inhibitory. |
| Databáze: | OpenAIRE |
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