Protection of BALE/c mice from respiratory syncytial virus infection by immunization with a synthetic peptide derived from the G glycoprotein
| Autor: | Cécile Séguin, H. Binz, Michel Trudel, Francine Nadon |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Paramyxoviridae
medicine.drug_class Enzyme-Linked Immunosorbent Assay Monoclonal antibody Respirovirus Infections Virus Microbiology BALB/c Epitopes Mice Viral Proteins Viral Envelope Proteins Antigen Virology medicine Animals Humans Antigens Viral Mice Inbred BALB C Vaccines Synthetic HN Protein biology Viral Vaccines biology.organism_classification Peptide Fragments Respiratory Syncytial Viruses Kinetics Immunization Hemocyanins biology.protein Female Antibody Keyhole limpet hemocyanin |
| Zdroj: | Virology. 185:749-757 |
| ISSN: | 0042-6822 |
| Popis: | A synthetic peptide homologous to amino acids 174-187 of the G glycoprotein of the A2 strain of human respiratory syncytial (RS) virus (G/174-187) was shown to induce protection from live virus challenge of BALB/c mice after immunization with three doses of 50 micrograms of peptide coupled to keyhole limpet hemocyanin. Immunized mice showed high levels of circulating RS-specific antibodies as detected by ELISA assay; however, no neutralizing antibodies were found. Moreover, an important short-term cytotoxic T-cell response was observed with lymphocytes isolated from the lungs but not from the spleen of immunized mice. This response was lost 24 weeks after immunization; however, mice remained protected against challenge with live RS virus. In addition, a monoclonal antibody that specifically binds to peptide G/174-187 was found efficient in conferring passive protection from challenge: this data further supports our results on the importance of the 174-187 region in protection. Another peptide, spanning amino acids 144 to 159, was shown to induce neutralizing antibodies but did not confer protection. |
| Databáze: | OpenAIRE |
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