Immunocytochemical characterization of viruses and antigenic macromolecules in viral vaccines
| Autor: | Nguyen Minh Lien, Nguyen Thanh Thuy, Nguyen Thu Van, Jean Cohen, Nguyen Kim Giao, Huynh Thi Phuong Lien, E. Lucio Benedetti, Nguyen Van Man, Irene Dunia, Hoang Thuy Nguyen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
viruses
CHO Cells Biology Antibodies Viral Transfection General Biochemistry Genetics and Molecular Biology Virus law.invention Microbiology Mice Immunolabeling Antigen law Cricetinae Animals Humans Encephalitis Japanese Microscopy Immunoelectron Antigens Viral Encephalitis Virus Japanese Hepatitis A Vaccines Hepatitis B Surface Antigens Ecology Japanese Encephalitis Vaccines Viral Vaccine Brain Viral Vaccines Immunogold labelling Hepatitis B Virology Recombinant Proteins GINS Poliovirus Vaccines Carrier State Recombinant DNA biology.protein Antibody |
| Zdroj: | Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie. 324:815-827 |
| ISSN: | 0764-4469 |
| DOI: | 10.1016/s0764-4469(01)01360-9 |
| Popis: | Gold immunolabeling combined with negative staining (GINS) provides a valuable immunocytochemical approach that allows a direct ultrastructural definition of all viral vaccine constituents that share common antigenic features with pathogenic viral particles. These results have implications for the development of viral vaccines since it has been demonstrated that incomplete viral particles such as natural empty capsides and Rotavirus-like particles lacking the infective genome are potential candidates for the production of neutralizing antibodies. Furthermore comparative results of the application of GINS to either inactivated vaccines or unfixed samples provide direct evidence that even after inactivation specific antigenic sites are still available for gold immunolabeling. |
| Databáze: | OpenAIRE |
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