In vivo Characterization of Brain Morphometric and Metabolic Endophenotypes in Three Inbred Strains of Mice Using Magnetic Resonance Techniques
| Autor: | Sylviane Confort-Gouny, Marie-France Penet, Angèle Viola, Patrick J. Cozzone, Yann Le Fur, Catherine Heurteaux, Christophe Laigle |
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| Přispěvatelé: | Centre de résonance magnétique biologique et médicale (CRMBM), Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS) |
| Rok vydání: | 2006 |
| Předmět: |
[SPI.OTHER]Engineering Sciences [physics]/Other
medicine.medical_specialty Neurology Hippocampus Mice Inbred Strains Biology Mice 03 medical and health sciences 0302 clinical medicine Inbred strain Genetic model Genetics medicine Animals Genetics (clinical) Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences medicine.diagnostic_test Brain Magnetic resonance imaging medicine.disease Magnetic Resonance Imaging 3. Good health Mice Inbred C57BL Metabolism Phenotype medicine.anatomical_structure Schizophrenia Endophenotype Neuroscience 030217 neurology & neurosurgery Neuroanatomy |
| Zdroj: | Behavior Genetics Behavior Genetics, Springer Verlag, 2006, 36 (5), pp.732-744 Behavior Genetics, 2006, 36 (5), pp.732-744 |
| ISSN: | 1573-3297 0001-8244 |
| DOI: | 10.1007/s10519-006-9077-4 |
| Popis: | C57BL6J, FVB/N and 129/SvJ mice are commonly used as background strains to engineer genetic models of brain pathologies and psychiatric disorders. Magnetic resonance imaging (MRI) and spectroscopy provide alternative approaches to neuroanatomy, histology and neurohistochemistry for investigating the correlation between genes and brain neuroanatomy and neurometabolism in vivo. We used these techniques to non-invasively characterize the cerebral morphologic and metabolic endophenotypes of inbred mouse strains commonly used in neurological and behavioral research. We observed a great variability in the volume of ventricles and of structures involved in cognitive function (cerebellum and hippocampus) among these strains. In addition, distinct metabolic profiles were evidenced with variable levels of N-acetylaspartate, a neuronal marker, and of choline, a compound found in membranes and myelin. Besides, significant differences in high-energy phosphates and phospholipids were detected. Our findings demonstrate the great morphologic and metabolic heterogeneity among C57BL/ 6J, FVB/N and 129/SvJ mice. They emphasize the importance of selecting the appropriate genetic background for over-expressing or silencing a gene and provide some directions for modeling symptoms that characterize psychiatric disorders such as autism, schizophrenia and depression. |
| Databáze: | OpenAIRE |
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