Role of prostaglandin E2 in peptidoglycan mediated iNOS expression in mouse peritoneal macrophages in vitro
| Autor: | Rajeev Kumar Pandey, Kunal H. Bhatt, Yogesh Dahiya, Ajit Sodhi |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Macrophage Prostaglandin E2 Biophysics Gene Expression Nitric Oxide Synthase Type II Peptidoglycan In Vitro Techniques Biochemistry Dinoprostone Mice chemistry.chemical_compound Paracrine signalling Structural Biology Gene expression Genetics Extracellular medicine Animals RNA Messenger RNA Small Interfering Inducible nitric oxide synthase Autocrine signalling Molecular Biology Mice Inbred BALB C Base Sequence biology Chemistry Cell Biology Cell biology Nitric oxide synthase Autocrine Communication Cyclooxygenase 2 Host-Pathogen Interactions Macrophages Peritoneal biology.protein Female medicine.drug |
| Zdroj: | FEBS Letters. 584:4227-4232 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2010.09.009 |
| Popis: | Many extracellular stimuli, e.g. microbial products, cytokines etc., result in the expression of inducible nitric oxide synthase (iNOS) in macrophages. However, it is not known whether expression of the iNOS gene in response to microbial products is a primary response of macrophages, or is the result of paracrine/autocrine signalling induced by endogenous biomolecules that are synthesised as a result of host cell–microbe interaction. In this paper we demonstrate that iNOS expression in mouse peritoneal macrophages in response to bacterial peptidoglycan (PGN) is a secondary effect requiring autocrine signalling of endogenously produced prostaglandin E2, and that PGN stimulation is mandatory, but not sufficient in itself, for induction of iNOS expression. |
| Databáze: | OpenAIRE |
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