Safeguarding Nonhuman Primate iPS Cells With Suicide Genes
| Autor: | Martin E. Wohlfahrt, Hans-Peter Kiem, Jennifer E. Adair, Korashon L. Watts, Jennifer L. Gori, Joerg Enssle, Bonan Zhong |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Cellular differentiation
Genetic Vectors Green Fluorescent Proteins Induced Pluripotent Stem Cells Mutagenesis (molecular biology technique) Mice SCID Cell fate determination Biology Regenerative Medicine Regenerative medicine Cell Line Insertional mutagenesis 03 medical and health sciences Mice 0302 clinical medicine Mice Inbred NOD Drug Discovery Genetics Animals Humans Cloning Molecular Induced pluripotent stem cell Molecular Biology 030304 developmental biology Cell Proliferation Pharmacology 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction Lentivirus Genes Transgenic Suicide Teratoma Cell Differentiation Sequence Analysis DNA Suicide gene 3. Good health Cell biology Blotting Southern Mutagenesis Insertional Gene Expression Regulation Cell culture 030220 oncology & carcinogenesis Models Animal Molecular Medicine Macaca Original Article |
| Popis: | The development of technology to generate induced pluripotent stem (iPS) cells constitutes one of the most exciting scientific breakthroughs because of the enormous potential for regenerative medicine. However, the safety of iPS cell-related products is a major concern for clinical translation. Insertional mutagenesis, possible oncogenic transformation of iPS cells or their derivatives, or the contamination of differentiated iPS cells with undifferentiated cells, resulting in the formation of teratomas, have remained considerable obstacles. Here, we demonstrate the utility of suicide genes to safeguard iPS cells and their derivatives. We found suicide genes can control the cell fate of iPS cells in vitro and in vivo without interfering with their pluripotency and self-renewal capacity. This study will be useful to evaluate the safety of iPS cell technology in a clinically highly relevant, large animal model and further benefit the clinical use of human iPS cells. |
| Databáze: | OpenAIRE |
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