Identification and isolation of putative stem cells from the murine placenta
| Autor: | Ross S. Basch, Bruce K. Young, Christine L Proudfit, Michael K Chan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Placenta
Population Cell Real-Time Polymerase Chain Reaction Mice Pregnancy Medicine Animals Progenitor cell education education.field_of_study business.industry Reverse Transcriptase Polymerase Chain Reaction Multipotent Stem Cells Mesenchymal stem cell Obstetrics and Gynecology Mesenchymal Stem Cells Hematopoietic Stem Cells Cell biology Haematopoiesis medicine.anatomical_structure Multipotent Stem Cell Pediatrics Perinatology and Child Health Antigens Surface Female Stem cell business Biomarkers Transcription Factors |
| Zdroj: | Journal of perinatal medicine. 42(6) |
| ISSN: | 1619-3997 |
| Popis: | The placenta of mid-gestation mice is a known rich source of hematopoietic stem cells. We hypothesized that it is also a source of other multipotent stem cells.We isolated fetal cells from the murine placenta across the second half of gestation and characterized their expression of surface antigens known to be associated with mesenchymal stem cells (MSCs) on a subset of hematopoietic lineage-negative cells. Using real-time reverse-transcriptase quantitative polymerase chain reaction, we also evaluated the expression of intracellular transcription factors (TFs) known to be associated with renal development and/or multipotent stem cells.Cell phenotypes with surface marker and TF expression consistent with multipotent stem cells of a mesenchymal lineage as well as renal cell progenitors were found in the placenta. The expression of MSC and renal progenitor surface markers varied throughout gestation, but was highest on E12–15 where such cells represented a small but significant percentage of the population. Of the studied TFs, 10 of 11 renal TFs were found at moderate to high levels, and all stem cell TFs were found.The mid-gestation murine placenta may serve as a source of multipotent stem cells and also contains cells which may be renal cell progenitors. |
| Databáze: | OpenAIRE |
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