Antibodies to Trophoblast Antigens HLA-G, Placenta Growth Factor, and NeuroD2 Do Not Improve Detection of Circulating Trophoblast Cells in Maternal Blood
| Autor: | Laurent Delli-Bovi, May Lee Tjoa, Diana W. Bianchi, Kirby L. Johnson |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Sex Determination Analysis Embryology Immunocytochemistry Gestational Age Prenatal diagnosis Cell Separation Pregnancy Proteins Biology Blood cell HLA Antigens Pregnancy Fetal membrane Prenatal Diagnosis Basic Helix-Loop-Helix Transcription Factors Centrifugation Density Gradient medicine Humans Radiology Nuclear Medicine and imaging In Situ Hybridization Fluorescence reproductive and urinary physiology Placenta Growth Factor HLA-G Antigens Fetus Histocompatibility Antigens Class I Neuropeptides Antibodies Monoclonal Obstetrics and Gynecology Trophoblast General Medicine medicine.disease Immunohistochemistry female genital diseases and pregnancy complications Trophoblasts medicine.anatomical_structure embryonic structures Pediatrics Perinatology and Child Health Immunology biology.protein Female Antibody |
| Zdroj: | Fetal Diagnosis and Therapy. 22:85-89 |
| ISSN: | 1421-9964 1015-3837 |
| DOI: | 10.1159/000097102 |
| Popis: | Objectives: Non-invasive prenatal diagnosis using circulating fetal trophoblast cells has been challenging due to lack of a reproducible trophoblast-specific antibody. We investigated the use of three trophoblast cell-specific antibodies, HLA-G, placenta growth factor, and neuroD2, for the isolation of trophoblast cells from the maternal circulation. Methods: Trophoblast cells were isolated by density centrifugation from maternal blood samples (gestational age 10–20 weeks, n = 9). All women were carrying a male fetus. Following immunocytochemical staining with the trophoblast-specific antibodies, fluorescent in situ hybridization was performed, to verify whether any stained cells were indeed fetal. Results: The HLA-G antibody had a ubiquitous staining pattern, which was not specific for trophoblast cells. Neither the placenta growth factor nor the neuroD2 antibodies were able to identify any trophoblast cells. Following fluorescent in situ hybridization, no male cells were detected on any of the slides. Conclusion: The antibodies used in this study were unable to improve detection of trophoblast cells in the maternal circulation. |
| Databáze: | OpenAIRE |
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