Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening
| Autor: | Deanpen Japrung, Wassa Waiwinya, Dechnarong Pimalai, Thitirat Putnin, Chuleekorn Chotsuwan, Noppadol Aroonyadet |
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| Rok vydání: | 2021 |
| Předmět: |
Silver
Nanoparticle Nanochemistry Metal Nanoparticles Breast Neoplasms Biosensing Techniques Analytical Chemistry law.invention law Limit of Detection Biomarkers Tumor Polyamines Humans Multiplex Detection limit Chromatography Chemistry Graphene Electrochemical Techniques MicroRNAs Linear range Colloidal gold Electrode Gold Antibodies Immobilized Porosity |
| Zdroj: | Mikrochimica actaReferences. 188(10) |
| ISSN: | 1436-5073 |
| Popis: | A highly sensitive electrochemical biosensors has been developed for the detection of multiplex micro ribonucleic acids (miRNAs) by modifying an electrode with reduced graphene oxide/poly(2-aminobenzylamine)/gold nanoparticles and adopting porous, hollow silver-gold nanoparticles as tagged labeling with metal ions. In addition, an anti-deoxyribonucleic acid (DNA)-RNA hybrid [S9.6] antibody was used to detect different hybridized capture DNAs and miRNAs that can detect multiple miRNAs simultaneously. The developed electrochemical platform exhibits high selectivity, stability, and sensitivity with a wide linear range from 1 fM to 10 nM and a low detection limit of 0.98 fM, 3.58 fM, and 0.25 fM for miRNA-155, miRNA-21, and miRNA-16, respectively. In addition, the proposed electrochemical biosensor capable for the simultaneous detection of miRNA-155, miRNA-16, and miRNA-21, which are breast cancer biomarkers, in normal human serum, can be adopted and potentially used for breast cancer screening. |
| Databáze: | OpenAIRE |
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