Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ10 Deficiency
| Autor: | Kate Duberley, Robert K. Heaton, Iain P. Hargreaves |
|---|---|
| Přispěvatelé: | guest, P |
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Coenzyme Q10 RM Antioxidant Chemistry medicine.medical_treatment Cell model Energy metabolism 030105 genetics & heredity Cell biology 03 medical and health sciences Antioxidant capacity chemistry.chemical_compound 030104 developmental biology Mitochondrial respiratory chain Research studies medicine |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071604700 |
| Popis: | Coenzyme Q10 (CoQ10) plays a key role as an electron carrier in the mitochondrial respiratory chain and as a cellular antioxidant molecule. A deficit in CoQ10 status may contribute to disease pathophysiology by causing a failure mitochondrial energy metabolism as well as compromising cellular antioxidant capacity. This chapter outlines the analytical methods used for determining cellular CoQ10 status using high-pressure liquid chromatography with ultraviolet (HPLC-UV) detection. In addition, we present a pharmacological procedure for establishing a human neuronal cell model of CoQ10 deficiency, for use in research studies. |
| Databáze: | OpenAIRE |
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