Transcriptome analysis of differentiating spermatogonia stimulated with kit ligand
| Autor: | Raffaele Geremia, Francesca Lolicato, Susanna Dolci, Paola Grimaldi, Annarita Di Sauro, Doria Filipponi, Pellegrino Rossi |
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| Rok vydání: | 2008 |
| Předmět: |
Male
DNA Complementary Transcription Genetic Cells Cell Stem cell factor Biology RNA Complementary Transcriptome Mice Genetic Meiosis Cells Cultured RNA Genome Oligonucleotide Array Sequence Analysis Spermatogonia Animals Stem Cell Factor Proto-Oncogene Proteins c-kit Cell Differentiation Gene Expression Regulation Developmental Cell Cycle Complementary Gene expression Genetics medicine Developmental Molecular Biology Mitosis Gene Settore BIO/16 Cultured urogenital system DNA Cell cycle Molecular biology medicine.anatomical_structure Gene Expression Regulation Transcription Developmental Biology |
| Zdroj: | Gene Expression Patterns. 8:58-70 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.modgep.2007.10.007 |
| Popis: | Kit ligand (KL) is a survival factor and a mitogenic stimulus for differentiating spermatogonia. However, it is not known whether KL also plays a role in the differentiative events that lead to meiotic entry of these cells. We performed a wide genome analysis of difference in gene expression induced by treatment with KL of spermatogonia from 7-day-old mice, using gene chips spanning the whole mouse genome. The analysis revealed that the pattern of RNA expression induced by KL is compatible with the qualitative changes of the cell cycle that occur during the subsequent cell divisions in type A and B spermatogonia, i.e. the progressive lengthening of the S phase and the shortening of the G2/M transition. Moreover, KL up-regulates in differentiating spermatogonia the expression of early meiotic genes (for instance: Lhx8, Nek1, Rnf141, Xrcc3, Tpo1, Tbca, Xrcc2, Mesp1, Phf7, Rtel1), whereas it down-regulates typical spermatogonial markers (for instance: Pole, Ptgs2, Zfpm2, Egr2, Egr3, Gsk3b, Hnrpa1, Fst, Ptch2). Since KL modifies the expression of several genes known to be up-regulated or down-regulated in spermatogonia during the transition from the mitotic to the meiotic cell cycle, these results are consistent with a role of the KL/kit interaction in the induction of their meiotic differentiation. |
| Databáze: | OpenAIRE |
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