Chemical Biology Tools for Examining the Bacterial Cell Wall
| Autor: | Stephen N. Hyland, Rebecca A. Gordon, M. Sloan Siegrist, Catherine L. Grimes, Ashley R Brown |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Magnetic Resonance Spectroscopy
Clinical Biochemistry Cell Chemical biology Peptidoglycan Computational biology Biology 01 natural sciences Biochemistry Article Bacterial cell structure Small Molecule Libraries Cell wall Metabolic engineering Bacterial Proteins Cell Wall Drug Discovery medicine Phospholipid Transfer Proteins Peptidoglycan biosynthesis Molecular Biology Pharmacology Bacteria 010405 organic chemistry Anti-Bacterial Agents 0104 chemical sciences medicine.anatomical_structure Isotope Labeling Molecular Medicine Biogenesis |
| Zdroj: | Cell Chem Biol |
| ISSN: | 2451-9456 |
| DOI: | 10.1016/j.chembiol.2020.07.024 |
| Popis: | Bacteria surround themselves with cell walls to maintain cell rigidity and protect against environmental insults. Here we review chemical and biochemical techniques employed to study bacterial cell wall biogenesis. Recent advances including the ability to isolate critical intermediates, metabolic approaches for probe incorporation, and isotopic labeling techniques have provided critical insight into the biochemistry of cell walls. Fundamental manuscripts that have used these techniques to discover cell wall-interacting proteins, flippases, and cell wall stoichiometry are discussed in detail. The review highlights that these powerful methods and techniques have exciting potential to identify and characterize new targets for antibiotic development. |
| Databáze: | OpenAIRE |
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