Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction
| Autor: | Markus Gorfer, Pál Vági, Márk Z. Németh, Levente Kiss, Gábor M. Kovács, Alexandra Pintye, Áron Horváth |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypha Agrobacterium Green Fluorescent Proteins Biological pest control Plant Science Biology 01 natural sciences Green fluorescent protein 03 medical and health sciences Ascomycota Botany Plant Diseases Obligate fungi food and beverages Plants biology.organism_classification Luminescent Proteins Transformation (genetics) 030104 developmental biology Agrobacterium tumefaciens Phyllosphere Agronomy and Crop Science Powdery mildew 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 109:1404-1416 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-01-19-0013-r |
| Popis: | Powdery mildews, ubiquitous obligate biotrophic plant pathogens, are often attacked in the field by mycoparasitic fungi belonging to the genus Ampelomyces. Some Ampelomyces strains are commercialized biocontrol agents of crop pathogenic powdery mildews. Using Agrobacterium tumefaciens-mediated transformation (ATMT), we produced stable Ampelomyces transformants that constitutively expressed green fluorescent protein (GFP) to (i) improve the visualization of the mildew–Ampelomyces interaction and (ii) decipher the environmental fate of Ampelomyces fungi before and after acting as a mycoparasite. Detection of Ampelomyces structures, and especially hyphae, was greatly enhanced when diverse powdery mildew, leaf, and soil samples containing GFP transformants were examined with fluorescence microscopy compared with brightfield and differential interference contrast optics. We showed for the first time, to our knowledge, that Ampelomyces strains can persist up to 21 days on mildew-free host plant surfaces, where they can attack powdery mildew structures as soon as these appear after this period. As saprobes in decomposing, powdery mildew-infected leaves on the ground and also in autoclaved soil, Ampelomyces strains developed new hyphae but did not sporulate. These results indicate that Ampelomyces strains occupy a niche in the phyllosphere where they act primarily as mycoparasites of powdery mildews. Our work has established a framework for a molecular genetic toolbox for the genus Ampelomyces using ATMT. |
| Databáze: | OpenAIRE |
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