The Comprehensive Mouse Radiation Hybrid Map Densely Cross-Referenced to the Recombination Map: A Tool to Support the Sequence Assemblies
| Autor: | Janan T. Eppig, Mary Barter, Jennifer A. Kelmenson, Lucy B. Rowe |
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| Rok vydání: | 2002 |
| Předmět: |
Genetic Markers
X Chromosome Sequence assembly Animals Wild Mice Inbred Strains Computational biology Biology Genome Chromosomes Contig Mapping Set (abstract data type) Mice Cricetinae Gene Order Genetics Animals Ensembl Radiation hybrid mapping Crosses Genetic Genetics (clinical) Recombination Genetic Radiation Hybrid Mapping Resolution (electron density) DNA Resources Mice Inbred C57BL Recombination |
| Zdroj: | Genome Research. 13:122-133 |
| ISSN: | 1549-5469 1088-9051 |
| DOI: | 10.1101/gr.858103 |
| Popis: | We have developed a unique comprehensive mouse radiation hybrid (RH) map of nearly 23,000 markers integrating data from three international genome centers and over 400 independent laboratories. We have cross-referenced this map to the 0.5-cM resolution recombination-based Jackson Laboratory (TJL) backcross panel map, building a complete set of RH framework chromosome maps based on a high density of known-ordered anchor markers. We have systematically typed markers to improve coverage and resolve discrepancies, and have reanalyzed data sets as needed. The cross-linking of the RH and recombination maps has resulted in a highly accurate genome-wide map with consistent marker order. We have compared these linked framework maps to the Ensembl mouse genome sequence assembly, and show that they are a useful medium resolution tool for both validating sequence assembly and elucidating chromosome biology.[Supplemental material is available online at www.genome.org.] |
| Databáze: | OpenAIRE |
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