Serological and molecular characterization of Leptospira kirschneri serogroup Grippotyphosa isolated from bovine in Brazil
| Autor: | Mayara Mafra Soares, Karla R. Lemes, Fernando P. Macedo, Anna Monteiro Correia Lima, Lauren Hubert Jaeger, Walter Lilenbaum, Dayane Olímpia Gomes, Pollyanna Mafra Soares |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary medicine animal diseases 030106 microbiology Cattle Diseases Context (language use) Serogroup Microbiology 03 medical and health sciences Leptospira Direct agglutination test medicine Animals Leptospirosis Public Health Surveillance Genotyping biology business.industry biology.organism_classification medicine.disease Molecular Typing 030104 developmental biology Infectious Diseases Leptospira kirschneri Herd Cattle Livestock business Brazil |
| Zdroj: | Microbial Pathogenesis. 138:103803 |
| ISSN: | 0882-4010 |
| Popis: | Leptospirosis is a zoonotic disease caused by a bacterium of the genus Leptospira that affects domestic animals, including cattle. Despite of this importance in livestock, little is known about the epidemiology of leptospirosis in cattle in Brazil and other tropical countries. In this context, the objective of this study was to perform the culture of urine and kidney (n = 372 animals) of slaughtered bovines of Minas Gerais state (Brazil), in order to obtain pure cultures of Leptospira strains and carry out its characterization by serological (microscopic agglutination test) and molecular techniques (rrs, secY sequencing and variable-number tandem-repeat genotyping). One strain, named UFU 02, was obtained from a male bovine without apparent alterations in ante-mortem examination. The strain was characterized belonging to species Leptospira kirschneri and serogroup Grippotyphosa. The serogroup Grippotyphosa is most often described in wild species, suggesting a link between domestic and wild cycles/environments. Considering the importance of the country in livestock breeding, the knowledge about leptospiral strains could help to control the disease in the herds. |
| Databáze: | OpenAIRE |
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