Divergent Effects of Retinoic Acids on the Expression of ERα and 17β-Hydroxysteroid Dehydrogenase Type 2 in Endometrial Carcinoma Cells (RL 95-2)
| Autor: | Yun-shang Piao, Xiao-Hong Li, Hui Li, Zhi-jie Xiao |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
17-Hydroxysteroid Dehydrogenases
Receptors Retinoic Acid Endocrinology Diabetes and Metabolism Clinical Biochemistry Retinoic acid Tretinoin Biology Retinoid X receptor Biochemistry chemistry.chemical_compound Endocrinology Reference Values In vivo Gene expression Estrogen Receptor beta Humans RNA Messenger Hydroxysteroid dehydrogenase Cells Cultured Messenger RNA Cell growth Carcinoma Biochemistry (medical) Estrogen Receptor alpha Molecular biology Endometrial Neoplasms Isoenzymes Retinoid X Receptors Receptors Estrogen chemistry Cell culture Female Oxidation-Reduction Cell Division Transcription Factors |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 87:640-649 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.87.2.8208 |
| Popis: | The effects of E2 are dependent on ERs and local E2 concentration in target cells. Modulation of intracellular E2 concentration involves the action of 17beta-hydroxysteroid dehydrogenase (17HSD) type 2, the enzyme converting E2 to estrone. In the present study, the influence of RAs on the growth of endometrial cancer cell line RL 95-2 as well as the expression of ERs and 17HSD type 2 have been investigated. It was found that RAs repress the growth of RL 95-2 cells, which express all subtypes of RXR and RAR, as examined by RT-PCR. Also, quantitative RT-PCR analysis showed that both ERalpha and ERbeta are present in RL 95-2 cells, and Western blot assay further revealed that ERalpha expression was decreased by all trans-RA treatment. In contrast, RAs induced 17HSD type 2 mRNA expression in a dose- and time-dependent fashion. This stimulatory effect was also detected at the level of in vivo oxidative 17HSD activity in cultured cells. On the other hand, the abundance of 17HSD type 2 mRNA was not altered by RAs in cultured normal epithelial cells isolated from human early- and late-secretory endometrium. The data indicate that RAs have an inhibitory effect on the growth of RL 95-2 cells and a cross-talk with the estrogen pathway in estrogen-responsive endometrial cancer cells. |
| Databáze: | OpenAIRE |
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