The Effects of Transdermally Delivered Oleanolic Acid on Malaria Parasites and Blood Glucose Homeostasis in P. berghei-Infected Male Sprague-Dawley Rats

Autor: Musa V. Mabandla, Cephas T. Musabayane, Happiness Sibiya
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Blood Glucose
Male
Glycogens
Physiology
Plasmodium berghei
medicine.medical_treatment
Syzygium
Glycobiology
lcsh:Medicine
Pharmacology
Parasitemia
Biochemistry
Rats
Sprague-Dawley
chemistry.chemical_compound
Endocrinology
Chloroquine
Blood plasma
Medicine and Health Sciences
Glucose homeostasis
Insulin
Homeostasis
lcsh:Science
Oleanolic acid
Protozoans
Multidisciplinary
Glycogen
Pharmaceutics
Malarial Parasites
Hematology
Blood Sugar
humanities
Body Fluids
Blood
Drug Therapy
Combination
Anatomy
medicine.drug
Research Article
Combination therapy
Transdermal Patch Drug Delivery
Blood sugar
Biology
Administration
Cutaneous
Blood Plasma
03 medical and health sciences
Antimalarials
medicine
Parasitic Diseases
Animals
Oleanolic Acid
Transdermal Drug Delivery
Diabetic Endocrinology
Plant Extracts
lcsh:R
Organisms
Biology and Life Sciences
Tropical Diseases
Parasitic Protozoans
Hormones
Malaria
Rats
030104 developmental biology
chemistry
Immunology
lcsh:Q
Drug Delivery
Zdroj: PLoS ONE, Vol 11, Iss 12, p e0167132 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: The present study investigated the effects of transdermally delivered oleanolic acid (OA) monotherapy and in combination with chloroquine (CHQ) on malaria parasites and glucose homeostasis of P. berghei-infected male Sprague-Dawley rats. Oral glucose test (OGT) responses to OA-pectin patch and CHQ-OA combination matrix patch were monitored in non-infected and infected rats. To evaluate the short-term effects of treatment, percentage parasitaemia, blood glucose, glycogen and plasma insulin were monitored in separate groups of animals treated with either OA-patch monotherapy or CHQ-OA combination pectin patch over a 21-days period. Animals treated with drug-free pectin and CHQ acted as untreated and treated positive controls, respectively. Infected control rats exhibited significantly increased parasitaemia which was accompanied by hypoglycaemia. Both OA monotherapy and CHQ-OA combination therapy reduced and cleared the malaria parasites within a period of 4 and 3 days, respectively. Compared to respective controls groups, OGT responses of animals treated with OA monotherapy or CHQ-OA combination therapy exhibited lower blood glucose levels at all time points. A once-off transdermal application of OA-patch or CHQ-OA combination patch significantly improved blood glucose concentrations inducing any changes in insulin concentration. Transdermal OA used as a monotherapy or in combination with CHQ is able to clear and reduce the malaria parasites within a shorter period of time without eliciting any adverse effects on glucose homeostasis of P. berghei-infected rats.
Databáze: OpenAIRE
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