Cytochrome P-450 and free radical generation in rat liver microsomes under the influence of prostaglandin E1
| Autor: | V. V. Sadovnichy, V. U. Buko |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Antioxidant Free Radicals Cytochrome medicine.medical_treatment Clinical Biochemistry Down-Regulation Biochemistry Acetone Superoxide dismutase Genetics medicine Animals Cytochrome c oxidase NADH NADPH Oxidoreductases Alprostadil Molecular Biology Ethanol biology Superoxide Dismutase Chemistry Cytochrome c peroxidase NADPH Oxidases Cytochrome P-450 CYP2E1 Rats Inbred Strains Hydrogen Peroxide Cell Biology NADPH oxidation Rats Starvation Phenobarbital Cytochrome P-450 CYP2B1 Luminescent Measurements Microsomes Liver biology.protein Microsome lipids (amino acids peptides and proteins) NADP medicine.drug |
| Zdroj: | IUBMB Life. 39:1177-1184 |
| ISSN: | 1521-6543 |
| DOI: | 10.1080/15216549600201362 |
| Popis: | There is evidence to suggest that the mechanism of antioxidant effect of prostaglandin E1 (PGE1) is due to decrease of radical species generation by cytochrome P-450 in rat liver microsomes. Chronic alcohol intoxication increased NADPH oxidation, cytochrome P-450 content and NADPH-stimulated chemoluminiscence of microsomes. Ethanol also raised superoxide dismutase (SOD) activity in microsomes. PGE1 decreased cytochrome P-450 content, normalized NADPH oxidation, NADPH-induced chemoluminiscence and SOD activity in the liver of alcohol-treated rats. PGE developed the similar effect after microsomal induction by both acetone combined with starvation and phenobarbital normalizing all the above parameters. Therefore, PGE1 affects on both, ethanol-inducible IIE1 and phenobarbital-inducible IIB1 isoforms. |
| Databáze: | OpenAIRE |
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