Noninvasive biomarkers identify eosinophilic esophagitis: A prospective longitudinal study in children
| Autor: | Jian Du, Kwang-Youn Kim, Matt L. Kleinjan, Katie Amsden, Steven J. Ackerman, Mary Ellen Riffle, Amir F. Kagalwalla, Ming Yu Wang, Mirna Chehade, Joshua B. Wechsler, Elizabeth Gray, Preeth Alumkal, Barry K. Wershil |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Immunology education Urine Eosinophil-Derived Neurotoxin Gastroenterology Article Atopy 03 medical and health sciences 0302 clinical medicine Internal medicine Biopsy medicine Immunology and Allergy Eosinophilia Humans Longitudinal Studies Prospective Studies Eosinophilic esophagitis Child Eosinophil cationic protein medicine.diagnostic_test business.industry Eosinophilic Esophagitis Eosinophil respiratory system medicine.disease Eosinophils 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Biomarker (medicine) medicine.symptom business Biomarkers |
| Zdroj: | Allergy |
| ISSN: | 1398-9995 |
| Popis: | BACKGROUND: Esophageal histology is critical for diagnosis and surveillance of disease activity in eosinophilic esophagitis (EoE). A validated noninvasive biomarker has not been identified. We aimed to determine the utility of blood and urine eosinophil-associated proteins to diagnose EoE and predict esophageal eosinophilia. METHODS: Blood and urine were collected from children undergoing endoscopy with biopsy. Absolute eosinophil count (AEC), plasma eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), major basic protein-1 (MBP-1), galectin-10 (CLC/GAL-10), Eotaxin-2 and Eotaxin-3, and urine osteopontin (OPN) and matrix metalloproteinase-9 (MMP-9) were determined. Differences were assessed between EoE and control, and with treatment response. The capacity to predict EoE diagnosis and esophageal eosinophil counts was assessed. RESULTS: Of 183 specimens were collected from 56 EoE patients and 15 non-EoE controls with symptoms of esophageal dysfunction; 33 EoE patients had paired pre-and post-treatment specimens. Plasma (CLC/GAL-10, ECP, EDN, Eotaxin-3, MBP-1) and urine (OPN) biomarkers were increased in EoE compared to control. A panel comprising CLC/GAL-10, Eotaxin-3, ECP, EDN, MBP-1, and AEC was superior to AEC alone in distinguishing EoE from control. AEC, CLC/GAL-10, ECP, and MBP-1 were significantly decreased in patients with esophageal eosinophil counts |
| Databáze: | OpenAIRE |
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