Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes
| Autor: | P. Marques Alves, Bernardo Abecasis, Sergey Yu. Luchkin, S.C. Rosa, J. B. Nunes, Henrique V. Almeida, Arianna Menciassi, Rose Cristina Veiga Gomes de Carvalho, Margarida Serra, L. Silva Ferreira, L. R. Monteiro, Leonardo Ricotti, P. Jorge Oliveira, P. José Gouveia, A. Leonidovitch Kholkin, F. Sofia Carvalho, R. Pires das Neves |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Scaffold business.product_category Contraction (grammar) Time Factors Hydrocarbons Fluorinated Piezoelectric materials Drug Evaluation Preclinical 02 engineering and technology Cell Communication Cardiac tissue engineering Extracellular matrix chemistry.chemical_compound Vasoconstrictor Agents Myocytes Cardiac Heptanol Cells Cultured Electrospun fibers Tissue Scaffolds 021001 nanoscience & nanotechnology Extracellular Matrix Mechanics of Materials 0210 nano-technology Caprolactone Layer (electronics) Anti-Arrhythmia Agents Materials science Vinyl Compounds Polyesters Biophysics Bioengineering Biomaterials Contractility 03 medical and health sciences Microfiber Animals Humans Rats Wistar Tissue Engineering Magnetic Phenomena Myocardial Contraction Cardiotoxicity Coculture Techniques Electric Stimulation Nanostructures Rats 030104 developmental biology chemistry Doxorubicin Ceramics and Composites Nanofilms business Biomedical engineering |
| Zdroj: | Biomaterials Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | The use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects of cardiac extracellular matrix while preserving the contractility of cardiomyocytes. The scaffold is based on a poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing for magnetic nanofilm) coated with a layer of piezoelectric (PIEZO) microfibers of poly(vinylidene fluoride-trifluoroethylene) (MNF+PIEZO). The nanofilm creates a flexible support for cell contraction and the aligned PIEZO microfibers deposited on top of the nanofilm creates conditions for cell alignment and electrical stimulation of the seeded cells. Our results indicate that MNF+PIEZO scaffold promotes rat and human cardiac cell attachment and alignment, maintains the ratio of cell populations overtime, promotes cell-cell communication and metabolic maturation, and preserves cardiomyocyte (CM) contractility for at least 12 days. The engineered cardiac construct showed high toxicity against doxorubicin, a cardiotoxic molecule, and responded to compounds that modulate CM contraction such as epinephrine, propranolol and heptanol. |
| Databáze: | OpenAIRE |
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