Telmisartan attenuates hydrogen peroxide-induced apoptosis in differentiated PC12 cells
| Autor: | Jin Hong Gong, Xiao Dan Qian, Xuan Ke, Hao Hong, Hui Ying Wu, Guan Tao Du, Jin Luo Cheng, Guang Jun Liu, Guo Liang Meng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Apoptosis medicine.disease_cause Biochemistry PC12 Cells 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Western blot Lactate dehydrogenase medicine Animals Telmisartan Hydrogen peroxide Cytotoxicity medicine.diagnostic_test Cell Differentiation Hydrogen Peroxide Angiotensin II Molecular biology Rats Oxidative Stress 030104 developmental biology chemistry Neurology (clinical) Angiotensin II Type 1 Receptor Blockers 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | Metabolic brain disease. 33(4) |
| ISSN: | 1573-7365 |
| Popis: | The present study investigated the protective actions of telmisartan, an angiotensin II type 1 receptor blocker (ARBs), against the cell apoptosis induced by exposure to hydrogen peroxide (H2O2) in differentiated PC12 cells. Preincubation of PC12 cells with telmisartan prevented H2O2-induced cytotoxicity as indicated by increased MTT (3,(4,5-dimethylthiazole-2-yl)2,5-diphenyl-tetrazolium bromide) reduction, decreased lactate dehydrogenase (LDH) release, and improved morphological changes. Hoechst 33,258 staining showed that telmisartan markedly reduced shrunken nuclei of the cells, and Western blot analysis indicated that telmisartan significantly attenuated caspase-3 activity, as indicated by decreased ratio of cleaved Caspase-3 to its precursor and increased ratio of Bcl-2/Bax. The present findings showed that telmisartan protected against cellular oxidative damages by inhibiting apoptotic response. |
| Databáze: | OpenAIRE |
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