Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I
| Autor: | Vivian Jiang, Brian McCarthy, Nilabh Shastri, Shelley R. Starck, Tao Pan, Mariana Pavon-Eternod, Sharanya Prasad |
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| Rok vydání: | 2012 |
| Předmět: |
RNA
Transfer Leu T-Lymphocytes Reading frame Antigen-Presenting Cells Codon Initiator Mice Transgenic Major histocompatibility complex Mice Eukaryotic translation Eukaryotic initiation factor MHC class I Chlorocebus aethiops Protein biosynthesis Animals Humans Peptide Chain Initiation Translational Genetics Antigen Presentation Multidisciplinary Hybridomas biology Histocompatibility Antigens Class I Translation (biology) Mice Inbred C57BL Protein Biosynthesis Transfer RNA COS Cells biology.protein HeLa Cells |
| Zdroj: | Science (New York, N.Y.). 336(6089) |
| ISSN: | 1095-9203 |
| Popis: | Noncanonical Pathway The textbook view of translation of messenger RNA to protein is that it is always initiated from open reading frames (ORFs) that begin with an AUG codon (encodes methionine) by an initiator methionine-bound transfer RNA (tRNA). There is evidence, however, that some polypeptides are produced from non–AUG-initiated ORFs. Starck et al. (p. 1719 ; see the Perspective by Dever ) used a variety of biochemical techniques to determine the underlying mechanism for such nontraditional translation initiation. Comparison of translation initiation from AUG-initiated ORFs with those beginning with leucine CUG-initiated ORFs revealed that cells can use an elongator Leu-tRNA to initiate translation at CUG codons. CUG-initiated peptides were presented by major histocompatibility class I molecules and could activate T cells. |
| Databáze: | OpenAIRE |
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