Glucose Oligosaccharide and Long-Chain Glucomannan Feed Additives Induce Enhanced Activation of Intraepithelial NK Cells and Relative Abundance of Commensal Lactic Acid Bacteria in Broiler Chickens
| Autor: | Meijerink, Nathalie, de Oliveira, Jean E, van Haarlem, Daphne A, Hosotani, Guilherme, Lamot, David M, Stegeman, J Arjan, Rutten, Victor P M G, Jansen, Christine A, dI&I RA-I&I I&I, Immunologie, FAH veterinaire epidemiologie, dFAH AVR, dFAH I&I |
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| Přispěvatelé: | dI&I RA-I&I I&I, Immunologie, FAH veterinaire epidemiologie, dFAH AVR, dFAH I&I |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Veterinary medicine
T cells Glucomannan NK cells In ovo Feed conversion ratio Article Microbiology broiler chickens innate adaptive IELs microbiota glucose oligosaccharide long-chain glucomannan in vitro- in ovo- in vivo screening 03 medical and health sciences chemistry.chemical_compound Immune system In vivo SF600-1100 Innate in ovo 030304 developmental biology 0303 health sciences Innate immune system General Veterinary biology Glucose oligosaccharide Microbiota 0402 animal and dairy science in vivo screening 04 agricultural and veterinary sciences Adaptive biology.organism_classification 040201 dairy & animal science veterinary(all) Lactic acid Broiler chickens chemistry In vitro- in ovo- in vivo screening Long-chain glucomannan In vitro Bacteria |
| Zdroj: | Veterinary Sciences, Vol 8, Iss 110, p 110 (2021) Veterinary Sciences Veterinary Sciences, 8(6) Veterinary Sciences, 8(6), 1. Multidisciplinary Digital Publishing Institute Veterinary Sciences; Volume 8; Issue 6; Pages: 110 Veterinary Sciences 8 (2021) 6 |
| ISSN: | 2306-7381 |
| Popis: | Restrictions on the use of antibiotics in the poultry industry stimulate the development of alternative nutritional solutions to maintain or improve poultry health. This requires more insight in the modulatory effects of feed additives on the immune system and microbiota composition. Compounds known to influence the innate immune system and microbiota composition were selected and screened in vitro, in ovo, and in vivo. Among all compounds, 57 enhanced NK cell activation, 56 increased phagocytosis, and 22 increased NO production of the macrophage cell line HD11 in vitro. Based on these results, availability and regulatory status, six compounds were selected for further analysis. None of these compounds showed negative effects on growth, hatchability, and feed conversion in in ovo and in vivo studies. Based on the most interesting numerical results and highest future potential feasibility, two compounds were analyzed further. Administration of glucose oligosaccharide and long-chain glucomannan in vivo both enhanced activation of intraepithelial NK cells and led to increased relative abundance of lactic acid bacteria (LAB) amongst ileum and ceca microbiota after seven days of supplementation. Positive correlations between NK cell subsets and activation, and relative abundance of LAB suggest the involvement of microbiota in the modulation of the function of intraepithelial NK cells. This study identifies glucose oligosaccharide and long-chain glucomannan supplementation as effective nutritional strategies to modulate the intestinal microbiota composition and strengthen the intraepithelial innate immune system. |
| Databáze: | OpenAIRE |
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