Epidemiological Survey for Brucella in Wildlife and Stray Dogs, a Cat and Rodents Captured on Farms
| Autor: | Byung-Il Yoon, Moon Her, Suk Chan Jung, Lam Quang Truong, Tae-Wook Hahn, Jung Taek Kim |
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| Rok vydání: | 2011 |
| Předmět: |
Veterinary medicine
Biovar Wildlife Animals Wild Rodentia Brucella Cat Diseases Brucellosis Serology Rodent Diseases Dogs Seroepidemiologic Studies Republic of Korea medicine Animals Dog Diseases General Veterinary biology business.industry Agriculture bacterial infections and mycoses medicine.disease biology.organism_classification 16S ribosomal RNA Isolation (microbiology) Cats Livestock business |
| Zdroj: | Journal of Veterinary Medical Science. 73:1597-1601 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.11-0222 |
| Popis: | Brucella infections in wildlife originate either from contact with infected livestock or from a natural sustainable reservoir in wildlife populations. As South Korea has set a goal of brucellosis eradication by 2013, it is necessary to determine the prevalence of Brucella in wildlife and wild rodents. This information will play an important role in the control of brucellosis. Because of the absence of prominent clinical signs, direct and indirect laboratory tests are essential for diagnosing brucellosis. In this study, tissue and blood samples were taken from wild animals, abandoned dogs, a cat and wild rodents, and they were tested for Brucella or Brucella-specific antibodies by isolation, PCR and serology. Results showed that 18.6% (33/177) of blood samples were positive by PCR, and 5.7% (11/194) were positive by C-ELISA. However, none of these samples yielded culturable bacteria. Of the tissue samples, 9.7% (8/82) were positive by PCR. Brucella was isolated from only one tissue culture from a Chinese water deer carcass. This Brucella species was identified as Brucella abortus biovar 1 by biotyping, 16S rRNA PCR and the Bruce-ladder PCR assay. In this study, we reported the prevalence of Brucella in wildlife, dogs, a cat and rodents by using serological and molecular methods, and we report the first isolation of B. abortus in wild Chinese water deer in South Korea. |
| Databáze: | OpenAIRE |
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