Recombinant canine adenovirus type 2 expressing rabbit hemorrhagic disease virus VP60 protein provided protection against RHD in rabbits
| Autor: | Xiaoliang Hu, Chuan-song Quan, Bo-tao Li, De-sheng Kong, Liandong Qu, Jiasen Liu, Qian Jiang, Zuo Yu, Dongchun Guo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hemorrhagic Disease Virus Rabbit Blotting Western Genetic Vectors Gene Expression Disease Adenoviruses Canine Biology Immunofluorescence Microbiology Virus Madin Darby Canine Kidney Cells law.invention 03 medical and health sciences Dogs Western blot law medicine Animals Vector (molecular biology) Gene Polymerase chain reaction Caliciviridae Infections Viral Structural Proteins General Veterinary medicine.diagnostic_test Viral Vaccines General Medicine Virology Recombinant Proteins 030104 developmental biology Recombinant DNA Feasibility Studies Rabbits |
| Zdroj: | Veterinary Microbiology. 213:15-20 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2017.11.007 |
| Popis: | Rabbit hemorrhagic disease virus (RHDV) is responsible for rabbit hemorrhagic disease (RHD), which is an acute, lethal and highly contagious disease in both wild and domestic rabbits. Although current vaccines are highly effective for controlling RHD, they are derived from infected rabbit livers and their use is thus associated with safety and animal-welfare concerns. In this study, we generated a recombinant lentogenic canine adenovirus type 2 (CAV2) vector expressing the RHDV vp60 gene, named rCAV2-VP60. rCAV2-VP60 expressed VP60 protein in Madin-Darby canine kidney cells as demonstrated by western blot and immunofluorescence assay. Polymerase chain reaction confirmed that the vp60 gene was successfully inserted into rCAV2-VP60 and was still detectable after 20 passages, indicating its stable genetic character. We evaluated the feasibility of rCAV2-VP60 as a live-virus-vectored RHD vaccine in rabbits. rCAV2-VP60 significantly induced specific antibodies to RHDV and provided effective protection against RHDV lethal challenge. These results suggest that rCAV2 expressing RHDV VP60 could be a safe and efficient candidate vaccine against RHDV in rabbits. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect