| Autor: |
Victor A. Svyatchenko, Vladimir A. Ternovoi, Roman Y. Lutkovskiy, Elena V. Protopopova, Andrei S. Gudymo, Nataliya V. Danilchenko, Ivan M. Susloparov, Nataliya P. Kolosova, Alexander B. Ryzhikov, Oleg S. Taranov, Vladimir V. Omigov, Elena V. Gavrilova, Alexander P. Agafonov, Rinat A. Maksyutov, Valery B. Loktev |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Microorganisms; Volume 11; Issue 1; Pages: 180 |
| ISSN: |
2076-2607 |
| DOI: |
10.3390/microorganisms11010180 |
| Popis: |
In this study, we investigated the features of the infectious process by simulating co-infection with SARS-CoV-2 and human adenovirus type 5 (HAdV-5) or influenza A virus (IAV) in vitro and in vivo. The determination of infectious activity of viruses and digital PCR demonstrated that during simultaneous and sequential HAdV-5 followed by SARS-CoV-2 infection in vitro and in vivo, the HAdV-5 infection does not interfere with replication of SARS-CoV-2. The hamsters co-infected and mono-infected with SARS-CoV-2 exhibited nearly identical viral titers and viral loads of SARS-CoV-2 in the lungs. The hamsters and ferrets co-infected by SARS-CoV-2- and IAV demonstrated more pronounced clinical manifestations than mono-infected animals. Additionally, the lung histological data illustrate that HAdV-5 or IAV and SARS-CoV-2 co-infection induces more severe pathological changes in the lungs than mono-infection. The expression of several genes specific to interferon and cytokine signaling pathways in the lungs of co-infected hamsters was more upregulated compared to single infected with SARS-CoV-2 animals. Thus, co-infection with HAdV-5 or IAV and SARS-CoV-2 leads to more severe pulmonary disease in animals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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