The use of RANKL-coated brushite cement to stimulate bone remodelling
| Autor: | Jake E. Barralet, Uwe Gbureck, Svetlana V. Komarova, S. Adam Hacking, Damien Le Nihouannen |
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| Rok vydání: | 2008 |
| Předmět: |
Calcium Phosphates
musculoskeletal diseases Materials science Alginates Biophysics Osteoclasts Dentistry Bioengineering Monocytes Osseointegration Cell Line Bone remodeling Biomaterials Mice Glucuronic Acid Osteogenesis Osteoclast medicine Animals Brushite Cement biology business.industry Hexuronic Acids RANK Ligand Bone Cements Biomaterial Resorption Durapatite surgical procedures operative medicine.anatomical_structure Mechanics of Materials RANKL NIH 3T3 Cells Ceramics and Composites biology.protein Bone Remodeling business |
| Zdroj: | Biomaterials. 29:3253-3259 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2008.03.035 |
| Popis: | Calcium phosphate cements were first proposed as synthetic bone substitutes over two decades ago, however, they are characterised by slow chemical or cellular resorption and a slow osteointegration. In contrast, bone autograft has been shown to stimulate osteoclastogenesis and angiogenesis resulting in active bone remodelling and rapid graft incorporation. Therefore, we aimed to develop a biomaterial able to release a key stimulator of the bone remodelling process, cytokine RANKL. Cylinders of brushite cement, hydroxyapatite cement and sodium alginate were loaded with RANKL either by incorporation into the cement or by coating the material with soluble RANKL. To test the biological activity of these formulations, we assessed their effectiveness in inducing osteoclast formation from RAW 264.7 monocytic cell line. Only brushite and hydroxyapatite cements coated with RANKL allowed for retaining sufficient biological activity to induce osteoclast formation. Most efficient was coating 40 mg cylinder of brushite cement with 800 ng RANKL. We have found that RANKL-coated brushite cement exhibits osteoclastogenic activity for at least 1 month at 37 °C. Thus, we developed a formulation of brushite cement with RANKL – a synthetic bone graft that is similar to autografts in its ability to actively induce osteoclastogenesis. |
| Databáze: | OpenAIRE |
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