Marker rescue mapping of the combined Condit/Dales collection of temperature sensitive vaccinia virus mutants
| Autor: | Nicole E. Kay, Travis W. Bainbridge, Sayuri E.M. Kato, Nissin Moussatche, Alyson J. Brinker, Cindy Prins, Richard C. Condit, Susan M. D'Costa, Audra L. Strahl, Amber N. Shatzer |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Hot Temperature
Genes Viral viruses Mutant Vaccinia virus Viral Plaque Assay Biology medicine.disease_cause Genome Virus Article Cell Line 03 medical and health sciences chemistry.chemical_compound Virology Chlorocebus aethiops medicine Genetics Animals Gene Temperature sensitive mutant 030304 developmental biology 0303 health sciences Mutation 030302 biochemistry & molecular biology Genetic Complementation Test Chromosome Mapping Sequence Analysis DNA Temperature-sensitive mutant Complementation chemistry Mapping Poxvirus Vaccinia |
| Popis: | Complementation analysis of the combined Condit/Dales collection of vaccinia virus temperature-sensitive mutants has been reported (Lackner, C.A., D'Costa, S.M., Buck, C., Condit, R.C., 2003. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305, 240–259), however not all complementation groups have previously been assigned to single genes on the viral genome. We have used marker rescue to map at least one representative of each complementation group to a unique viral gene. The final combined collection contains 124 temperature-sensitive mutants affecting 38 viral genes, plus five double mutants. |
| Databáze: | OpenAIRE |
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