Parathyroid hormone mRNA levels are increased by progestins and vary during rat estrous cycle
| Autor: | G. Almogi, Nelly Livni, E. Epstein, Justin Silver, Tally Naveh-Many |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
endocrine system
medicine.medical_specialty Adenoma Physiology medicine.drug_class Ovariectomy Endocrinology Diabetes and Metabolism Gene Expression Parathyroid hormone Weaning Biology Promegestone Parathyroid Glands Rats Sprague-Dawley Estrus Physiology (medical) Internal medicine Progesterone receptor medicine Animals RNA Messenger Cells Cultured Progesterone Estrous cycle Rats Inbred Strains Parathyroid chief cell medicine.disease Rats medicine.anatomical_structure Endocrinology Parathyroid Hormone Estrogen Ovariectomized rat Cattle Female Parathyroid gland Progestins Receptors Progesterone hormones hormone substitutes and hormone antagonists |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 270:E158-E163 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1996.270.1.e158 |
| Popis: | Estrogen increases parathyroid hormone (PTH) mRNA levels in vivo in ovariectomized rats. We now show that the 19-norprogestin R-5020 given to weanling rats or mature ovariectomized rats led to a twofold increase in thyroparathyroid PTH mRNA levels. This increase in PTH mRNA occurred at 24 and 48 h after progesterone but not at 72 h. There were no changes in serum calcium. In vitro, in primary cultures of bovine parathyroid cells, progesterone increased PTH mRNA levels threefold at 10(-8) M and twofold at 10(-9) M after 24 h. Progesterone receptor (PR) mRNA was demonstrated in rat parathyroid tissue by in situ hybridization and in human parathyroid adenoma by immunohisto-chemistry. Changes in PTH mRNA levels during the rat estrous cycle were also studied. At proestrus and estrus PTH mRNA levels were increased significantly by three- and fourfold compared with diestrus. Our results confirm that the parathyroid gland is a target organ for the ovarian sex steroids estrogen and progesterone and are of physiological relevance as shown by the changes during estrus. |
| Databáze: | OpenAIRE |
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