Inhibition of cell migration and invasion by ICAM-1 binding DNA aptamers
| Autor: | Berke Bilgenur Şener, Ceren Bayraç, Abdullah Tahir Bayraç, Deniz Yiğit |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Aptamer
Biophysics 01 natural sciences Biochemistry Metastasis 03 medical and health sciences Circulating tumor cell Cricetulus Cell Movement medicine Animals Humans Molecular Biology Cells Cultured 030304 developmental biology 0303 health sciences ICAM-1 Binding Sites Chemistry 010401 analytical chemistry SELEX Aptamer Technique Cell migration Cell Biology Aptamers Nucleotide medicine.disease Flow Cytometry Intercellular Adhesion Molecule-1 Molecular biology 0104 chemical sciences Cancer cell Intracellular Systematic evolution of ligands by exponential enrichment |
| Zdroj: | Analytical biochemistry. 628 |
| ISSN: | 1096-0309 |
| Popis: | Cancer is the second leading cause of death worldwide and most of the cancer-related deaths result from metastasis. As expressed on the surface of various cancer cell types, intercellular adhesion molecule-1 (ICAM-1) has been shown to play a role in the attachment, invasion and migration of tumor cells. In this study, DNA aptamers were generated against ICAM-1 by cell-SELEX and protein SELEX method using ICAM-1(+) CHO-ICAM-1 cells and ICAM-1 protein, respectively. The pools obtained at the end of the 10th round of both SELEX were sequenced and the most enriched sequences were characterized for their binding behaviors and affinities to ICAM-1(+) CHO-ICAM-1 and ICAM-1(−) MIA PaCa-2 cells. Moreover, the inhibition abilities of sequences on migration and invasion were measured. The seven aptamer sequences were obtained selectively binding to CHO-ICAM-1 cells with Kd values in the ranging from 13.8 to 47.1 nM. Four of these aptamers showed inhibition in both migration and invasion of CHO-ICAM-1 cells at least 61%. All these results suggested that these aptamers have potential to detect specifically ICAM-1 expressing tumor cells and inhibit migration and invasion by blocking ICAM-1 related interactions of circulating tumor cells. |
| Databáze: | OpenAIRE |
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