Generation of CD8+ T cell-generated suppressor factor and beta-chemokines by targeted iliac lymph node immunization in rhesus monkeys challenged with SHIV-89.6P by the rectal route
| Autor: | O. Neildez, Françoise Barré-Sinoussi, Roger Le Grand, Christian Beyer, Anne-Marie Aubertin, Bruno Hurtrel, Marc Girard, Christiane Moog, Louisa Tao, Yufei Wang, Thomas Lehner |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Iliac Lymph Node animal diseases Immunology Simian Acquired Immunodeficiency Syndrome Gene Products gag chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes HIV Antibodies medicine.disease_cause Antibodies Viral Lymphocyte Activation Ilium Immune system Virology medicine Suppressor Factors Immunologic Cytotoxic T cell Animals Lymph node biology Rectum env Gene Products Human Immunodeficiency Virus Gene Products env biochemical phenomena metabolism and nutrition Simian immunodeficiency virus Viral Load Macaca mulatta Infectious Diseases medicine.anatomical_structure Chemokines CC Humoral immunity biology.protein bacteria Immunization Simian Immunodeficiency Virus Lymph Nodes Antibody CD8 |
| Zdroj: | AIDS research and human retroviruses. 16(4) |
| ISSN: | 0889-2229 |
| Popis: | The targeted lymph node (TLN) immunization strategy was investigated in macaques, in order to determine the efficacy in generating secretory, systemic, and cellular immune responses, CD8+ T cell-generated suppressor factors, and beta-chemokines. TLN immunization of the rectal and genital mucosa-associated iliac lymph nodes (TILNs) was compared with axillary TLN immunization (TAxLN) using HIV-1 MN/LAI gp140env and SIV p27gag in alum. Significantly higher immune responses, as well as CD8+ T cell-generated anti-SIV factors and the beta-chemokines RANTES, MIP-1alpha, and MIP-1beta, were elicited by iliac as compared with axillary TLN immunization. The immune responses induced by TLN immunization were examined for their capacity to prevent rectal mucosal infection by the pathogenic dual-tropic SHIV-89.6P. Despite significant secretory, serum, cellular, and beta-chemokine responses, the macaques were infected by SHIV-89.6P. Whether the lack of protection was associated with the antigenic unrelatedness of SHIV-89.6P to the immunizing HIV-1 MN/LAI gp140 or to the virus utilizing CXCR4 to a much greater extent than CCR5, remains to be determined. |
| Databáze: | OpenAIRE |
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