Apoptosis and proliferation in lungs of ventilated and oxygen-treated preterm infants
| Autor: | P Ströbel, A Marx, S Seidenspinner, T Preisshofen, Christian P. Speer, M May |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pulmonary and Respiratory Medicine
Programmed cell death Apoptosis Caspase 3 Caspase 8 Andrology In Situ Nick-End Labeling Humans Medicine Respiratory system Fetal Death Lung Respiratory Distress Syndrome Newborn Respiratory distress business.industry Infant Newborn Endothelial Cells Epithelial Cells Muscle Smooth Pulmonary Surfactants Immunohistochemistry Respiration Artificial Caspase 9 Oxygen medicine.anatomical_structure Caspases Immunology Breathing business Infant Premature |
| Zdroj: | European Respiratory Journal. 23:113-121 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.03.00038403 |
| Popis: | Apoptosis and proliferation and the effect of exogenous surfactant on these processes were investigated in the lungs of mechanically ventilated/oxygen-treated preterm infants with respiratory distress syndrome and stillborn foetuses. Apoptotic and proliferation indices were determined in lung tissue sections from 27 ventilated/oxygen-treated preterm infants and 29 stillborn foetuses. The effect of exogenous surfactant on apoptosis and proliferation was studied in 16 ventilated preterm infants; 11 untreated infants served as control. Apoptotic and proliferating cells were identified by double labelling combining terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick end-labelling or Ki-67 with cell marker proteins. Pathways to cell death were explored by immunolabelling of cleaved caspases-3, -8 and -9. In the lungs of ventilated/oxygen-treated preterm infants, the numbers of apoptotic and proliferating cells increased significantly compared to the respective numbers in the lungs of stillborn foetuses. Apoptosis was detected in alveolar epithelial cells, whereas epithelial, endothelial and smooth muscle cells proliferated. Surfactant treatment reduced apoptosis induced by ventilation/oxygen-treatment; however, the decrease was not significant. Caspases-8 and -9 do not contribute to ventilation-induced apoptosis, whereas caspase-3 is involved. In conclusion, ventilation/oxygen-treatment induces epithelial cell apoptosis and proliferation of epithelial, endothelial and smooth muscle cells in the lungs of preterm infants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|