The influence of IS1301 in the capsule biosynthesis locus on meningococcal carriage and disease

Autor: Ray Borrow, Holly B. Bratcher, Bridget Gollan, Ana Belén Ibarz-Pavón, Jay Lucidarme, Christoph M. Tang, Elisabeth Kugelberg, Christopher Farrance, Martin C. J. Maiden
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Bacterial capsule
Infectious Diseases/Epidemiology and Control of Infectious Diseases
DNA
Bacterial
lcsh:Medicine
Meningococcal Vaccines
Meningococcal vaccine
Neisseria meningitidis
Serogroup C
Biology
Neisseria meningitidis
Serogroup B
Meningococcal disease
medicine.disease_cause
Microbiology
law.invention
Infectious Diseases/Bacterial Infections
03 medical and health sciences
Bacterial Proteins
law
Sequence Homology
Nucleic Acid
Genotype
medicine
Humans
lcsh:Science
Polymerase chain reaction
Bacterial Capsules
Phylogeny
030304 developmental biology
0303 health sciences
Multidisciplinary
Base Sequence
030306 microbiology
Neisseria meningitidis
lcsh:R
Complement System Proteins
medicine.disease
Virology
United Kingdom
3. Good health
Meningococcal Infections
Mutagenesis
Insertional
Carriage
Spain
DNA Transposable Elements
DNA
Intergenic
lcsh:Q
Vaccine failure
Research Article
Zdroj: PLoS ONE, Vol 5, Iss 2, p e9413 (2010)
PLoS ONE
Popis: Previously we have shown that insertion of IS1301 in the sia/ctr intergenic region (IGR) of serogroup C Neisseria meningitidis (MenC) isolates from Spain confers increased resistance against complement-mediated killing. Here we investigate the significance of IS1301 in the same location in N. meningitidis isolates from the UK. PCR and sequencing was used to screen a collection of more than 1500 meningococcal carriage and disease isolates from the UK for the presence of IS1301 in the IGR. IS1301 was not identified in the IGR among vaccine failure strains but was frequently found in serogroup B isolates (MenB) from clonal complex 269 (cc269). Almost all IS1301 insertions in cc269 were associated with novel polymorphisms, and did not change capsule expression or resistance to human complement. After excluding sequence types (STs) distant from the central genotype within cc269, there was no significant difference for the presence of IS1301 in the IGR of carriage isolates compared to disease isolates. Isolates with insertion of IS1301 in the IGR are not responsible for MenC disease in UK vaccine failures. Novel polymorphisms associated with IS1301 in the IGR of UK MenB isolates do not lead to the resistance phenotype seen for IS1301 in the IGR of MenC isolates.
Databáze: OpenAIRE
Externí odkaz: