Starch-Capped AgNPs' as Potential Cytotoxic Agents against Prostate Cancer Cells
| Autor: | Gabriela Martins, Magda Fonseca, João A.V. Prior, Francisca Dias, Vera Machado, Ana Teixeira, Rui Medeiros, Carlos Palmeira, Mariana Morais, Catarina Martins |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
silver nanoparticles
Cell cycle checkpoint General Chemical Engineering Silver nanoparticle Article lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound anticancer agents 0302 clinical medicine LNCaP General Materials Science Propidium iodide Cytotoxicity 030304 developmental biology 0303 health sciences green synthesis starch Cell cycle prostate cancer lcsh:QD1-999 chemistry Cell culture 030220 oncology & carcinogenesis Biophysics cytotoxicity Trypan blue |
| Zdroj: | Nanomaterials Nanomaterials, Vol 11, Iss 256, p 256 (2021) Volume 11 Issue 2 |
| ISSN: | 2079-4991 |
| Popis: | One of the major therapeutic approaches of prostate cancer (PC) is androgen deprivation therapy (ADT), but patients develop resistance within 2&ndash 3 years, making the development of new therapeutic approaches of great importance. Silver nanoparticles (AgNPs) synthesized through green approaches have been studied as anticancer agents because of their physical-chemical properties. This study explored the cytotoxic capacity of starch-capped AgNPs, synthesized through green methods, in LNCaP and in PC-3 cells, a hormonal-sensitive and hormone-resistant PC cell line, respectively. These AgNPs were synthesized in a microwave pressurized synthesizer and characterized by ultraviolet-visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). Their cytotoxicity was assessed regarding their ability to alter morphological aspect (optical microscopy), induce damage in cytoplasmic membrane (Trypan Blue Assay), mitochondria (WST-1 assay), cellular proliferation (BrdU assay), and cell cycle (Propidium iodide and flow-cytometry). AgNPs showed surface plasmon resonance (SPR) of approximately 408 nm and average size of 3 nm. The starch-capped AgNPs successfully induced damage in cytoplasmic membrane and mitochondria, at concentrations equal and above 20 ppm. These damages lead to cell cycle arrest in G0/G1 and G2/M, blockage of proliferation and death in LNCaP and PC-3 cells, respectively. This data shows these AgNPs&rsquo potential as anticancer agents for the different stages of PC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|