The Role of Ames Dwarfism and Calorie Restriction on Gut Microbiota
| Autor: | Berta Victoria, Khalid A. Al-Regaiey, Till Strowig, Augusto Schneider, Lina Spinel, Karl-Herbert Schäfer, Brittany Allen, Eric J. C. Gálvez, Adam Gesing, Denise S. Wiesenborn, Michal M. Masternak |
|---|---|
| Přispěvatelé: | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine THE JOURNAL OF GERONTOLOGY: Biological Sciences Aging media_common.quotation_subject Longevity Calorie restriction Physiology Dwarfism Ileum Gut flora Mice 03 medical and health sciences Cecum 0302 clinical medicine Genotype Animals Medicine Microbiome Caloric Restriction media_common biology business.industry biology.organism_classification medicine.disease Mice Mutant Strains Gastrointestinal Microbiome 030104 developmental biology medicine.anatomical_structure Growth Hormone Models Animal Female Geriatrics and Gerontology business 030217 neurology & neurosurgery |
| Zdroj: | J Gerontol A Biol Sci Med Sci The journals of gerontology. Series A, Biological sciences and medical sciences |
| ISSN: | 1758-535X 1079-5006 |
| DOI: | 10.1093/gerona/glz236 |
| Popis: | The gut microbiome (GM) represents a large and very complex ecosystem of different microorganisms. There is an extensive interest in the potential role of the GM in different diseases including cancer, diabetes, cardiovascular diseases, and aging. The GM changes over the lifespan and is strongly associated with various age-related diseases. Ames dwarf (df/df) mice are characterized by an extended life- and healthspan, and although these mice are protected from many age-related diseases, their microbiome has not been studied. To determine the role of microbiota on longevity animal models, we investigated the changes in the GM of df/df and normal control (N) mice, by comparing parents before mating and littermate mice at three distinct time points during early life. Furthermore, we studied the effects of a 6-month calorie restriction (CR), the most powerful intervention extending the lifespan. Our data revealed significant changes of the GM composition during early life development, and we detected differences in the abundance of some bacteria between df/df and N mice, already in early life. Overall, the variability of the microbiota by genotype, time-point, and breeding pair showed significant differences. In addition, CR caused significant changes in microbiome according to gastrointestinal (GI) location (distal colon, ileum, and cecum), genotype, and diet. However, the overall impact of the genotype was more prominent than that of the CR. In conclusion, our findings suggest that the gut microbiota plays an important role during postnatal development in long-living df/df mice and CR dietary regimen can significantly modulate the GM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|