Whither the replisome: Emerging perspectives on the dynamic nature of the DNA replication machinery
| Autor: | Lance D. Langston, Chiara Indiani, Mike O'Donnell |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA Replication
Genetics DNA Repair Semiconservative replication DNA replication Eukaryotic DNA replication DNA-Directed DNA Polymerase Cell Biology Computational biology Biology Pre-replication complex Models Biological Article Minichromosome maintenance Control of chromosome duplication Multienzyme Complexes Escherichia coli Replisome Origin recognition complex DnaB Helicases Molecular Biology Developmental Biology |
| Zdroj: | Cell Cycle. 8:2686-2691 |
| ISSN: | 1551-4005 1538-4101 |
| DOI: | 10.4161/cc.8.17.9390 |
| Popis: | Replisomes were originally thought to be multi-protein machines with a stabile and defined structure during replication. Discovery that replisomes repeatedly discard sliding clamps and assemble a new clamp to start each Okazaki fragment provided the first hint that the replisome structure changes during replication. Recent studies reveal that the replisome is more dynamic than ever thought possible. Replisomes can utilize many different polymerases; the helicase is regulated to travel at widely different speeds; leading and lagging strands need not always act in a coupled fashion with DNA loops; and the replication fork does not always exhibit semi-discontinuous replication. We review some of these findings here and discuss their implications for cell physiology as well as enzyme mechanism. |
| Databáze: | OpenAIRE |
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