Characterization of the Nonstructural Proteins of the Bocavirus Minute Virus of Canines
| Autor: | Loretta Sukhu, Ayushi Rai, David J. Pintel, Lisa R. Burger, Olufemi O. Fasina, Jianming Qiu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
viruses
RNA Splicing Molecular Sequence Data Immunology Viral Nonstructural Proteins Microbiology Virus Cell Line Bocavirus Dogs Virology Animals Viral rna RNA Messenger Gene Messenger RNA Base Sequence biology Parvovirus biology.organism_classification Molecular biology Genome Replication and Regulation of Viral Gene Expression Capsid Cell culture Insect Science RNA splicing |
| Zdroj: | Journal of Virology. 87:1098-1104 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.02627-12 |
| Popis: | We present a detailed characterization of a single-cycle infection of the bocavirus minute virus of canines (MVC) in canine WRD cells. This has allowed identification of an additional smaller NS protein that derives from an mRNA spliced within the NS gene that had not been previously reported. In addition, we have identified a role for the viral NP1 protein during infection. NP1 is required for read-through of the MVC internal polyadenylation site and, thus, access of the capsid gene by MVC mRNAs. Although the mechanism of NP1's action has not yet been fully elucidated, it represents the first parvovirus protein to be implicated directly in viral RNA processing. |
| Databáze: | OpenAIRE |
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