A HPLC-UV method for the determination of puerarin in rat plasma after intravenous administration of PEGylated puerarin conjugate
| Autor: | Xinyi Liu, Jianrong Li, Wenjie Qin, Naijie Wang, Zuguang Ye, Hongying Zhi, Feng Du |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Clinical Biochemistry Sensitivity and Specificity Biochemistry High-performance liquid chromatography Polyethylene Glycols Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Drug Stability Pharmacokinetics Puerarin Spectrophotometry medicine Animals Phosphoric acid Chromatography High Pressure Liquid Detection limit Chromatography medicine.diagnostic_test Extraction (chemistry) Reproducibility of Results Cell Biology General Medicine Isoflavones Rats chemistry Benzaldehydes Injections Intravenous Linear Models Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B. 878:3297-3302 |
| ISSN: | 1570-0232 |
| Popis: | A sensitive and reproducible HPLC method for quantitative determination of puerarin (PUE) in rat plasma was developed and validated using 4-hydroxybenzaldehyde as an internal standard. The separation of PUE was performed on a CAPCELL PAK C18 column by gradient elution with 0.2% aqueous phosphoric acid and acetonitrile as the mobile phase. The method was validated and found to be linear in the range of 80-12,000ng/mL. The limit of quantification was 80ng/mL based on 100μL of plasma. The variations for intra- and inter-day precision were less than 8.3%, and the accuracy values were between 98% and 105.2%. The extraction recoveries were more than 85%. The method was successfully applied in the comparative study of pharmacokinetics of PEGylated puerarin (PEG-PUE) versus PUE in rats. Compared with PUE, PEG-PUE showed a 5.2-fold increase in half-life of PUE and a 4.7-fold increase in mean residence time. In addition, this method was also successfully applied to determine the low plasma concentration of PUE regenerated from PEG-PUE in vitro. |
| Databáze: | OpenAIRE |
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