Perkinsus olseni and P. chesapeaki detected in a survey of perkinsosis of various clam species in Galicia (NW Spain) using PCR-DGGE as a screening tool
| Autor: | Andrea Ramilo, Antonio Villalba, José Pintado, Elvira Abollo |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
In situ hybridisation Perkinsus olseni Zoology Ruditapes Biology Polymerase Chain Reaction 03 medical and health sciences Ruditapes decussatus Venerupis corrugata Species Specificity Ruditapes philippinarum Animals Perkinsus Polititapes rhomboides Ecology Evolution Behavior and Systematics In Situ Hybridization Phylogeny Likelihood Functions Ecology Sequence Analysis DNA biology.organism_classification Bivalvia PCR 030104 developmental biology Alveolata Spain |
| Zdroj: | Journal of invertebrate pathology. 133 |
| ISSN: | 1096-0805 |
| Popis: | 9 pages, 5 figures, 4 tables A survey on perkinsosis was performed involving 15 locations scattered along the Galician coast (NW Spain) and four clam species with high market value (Ruditapes decussatus, Ruditapes philippinarum, Venerupis corrugata and Polititapes rhomboides). The prevalence of Perkinsus parasites was estimated by PCR using genus-specific primers. The highest percentage of PCR-positive cases for perkinsosis corresponded to clams R. decussatus and V. corrugata, while lower values were detected in R. philippinarum and no case was found in P. rhomboides. The discrimination of Perkinsus species was performed by PCR–RFLP and by a new PCR–DGGE method developed in this study. Perkinsus olseni was identified in every clam species, except in P. rhomboides, using both PCR–DGGE and PCR–RFLP. Additionally, Perkinsus chesapeaki was only detected by PCR–DGGE infecting two Manila clams R. philippinarum from the same location, reporting the first case in Galicia. P. chesapeaki identification was further confirmed by in situ hybridisation assay and phylogenetic analysis of ITS region and LSU rDNA. This study was funded by the Ministerio de Economía y Competitividad of the Government of Spain, through the project AGL2011-30449-C02-01. |
| Databáze: | OpenAIRE |
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