Plasma Arginine and Citrulline are Elevated in Diabetic Retinopathy
| Autor: | David C. Samuels, M. Wade Calcutt, Sabrina L. Mitchell, Milam A. Brantley, Katherine S. Peters, Emilio S. Rivera, Paula A. Harlow, Cassandra Warden |
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| Rok vydání: | 2022 |
| Předmět: |
Adult
medicine.medical_specialty Arginine Metabolite Type 2 diabetes Gastroenterology chemistry.chemical_compound Tandem Mass Spectrometry Internal medicine medicine Citrulline Humans Creatinine Diabetic Retinopathy business.industry Diabetic retinopathy Ornithine medicine.disease eye diseases Ophthalmology Cross-Sectional Studies Diabetes Mellitus Type 2 chemistry Asymmetric dimethylarginine business Chromatography Liquid |
| Zdroj: | American Journal of Ophthalmology. 235:154-162 |
| ISSN: | 0002-9394 |
| Popis: | Purpose To determine if plasma levels of six arginine and citrulline-related metabolites (arginine, citrulline, asymmetric dimethylarginine [ADMA], ornithine, proline, and argininosuccinate) differ between patients with type 2 diabetes and diabetic retinopathy (DR) and type 2 diabetic controls or between patients with proliferative DR (PDR) and non-proliferative DR (NPDR). Design Cross-sectional study Methods Adults with type 2 diabetes were recruited from the Vanderbilt Eye Institute. Exclusion criteria included non-diabetic retinal disease. Plasma metabolite levels were quantified in 159 diabetic controls and 156 DR patients (92 NPDR, 64 PDR) using isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Metabolite levels were compared using Wilcoxon Rank Sum test and logistic regressions adjusting for age, sex, hemoglobin A1c, diabetes duration, statin use, and angiotensin-converting enzyme inhibitor or angiotensin II receptor blocker use. A secondary analysis that included creatinine in the regression model was performed for the subset of patients with available creatinine values (135 diabetic controls, 100 DR patients [58 NPDR, 42 PDR]). Results Multivariable logistic regression analyses determined arginine (OR = 1.20, [1.06-1.38], P = 0.0067) and citrulline (OR = 1.53, [1.20-1.98], P = 0.0025) were significantly elevated in DR patients compared to diabetic controls. While ADMA differed between NPDR and PDR patients in the primary analysis (OR = 1.56, [1.15-2.16], P = 0.0051), it was not significantly different when adjusting for creatinine (OR = 1.30, [0.90-1.91], P = 0.15). Conclusions Plasma arginine and citrulline were significantly elevated in type 2 diabetic patients with DR compared to diabetic controls. None of the tested metabolites significantly differed between NPDR and PDR patients in the adjusted analysis. |
| Databáze: | OpenAIRE |
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